Differential biophysical properties of infectious intracellular and secreted hepatitis C virus particles
Differential biophysical properties of infectious intracellular and secreted hepatitis C virus particles
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DOI：
10.1128/jvi.01150-06
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发表时间：
2006-11-01
影响因子：
5.4
通讯作者：
Chisari, Francis V.
中科院分区：
文献类型：
--
作者：
Gastaminza, Pablo;Kapadia, Sharookh B.;Chisari, Francis V.
丙型肝炎病毒（HCV）细胞培养感染模型的最新发展允许在体外生产感染性颗粒。在本报告中，我们证明感染颗粒存在于感染细胞内和上清液中。动力学分析表明，细胞内颗粒构成分泌的感染性病毒的前体。超离心分析表明，细胞内感染性病毒颗粒大小相似（65 ~ 70 nm），但浮力密度（1.15 ~ 1.20 g/ml）与细胞外颗粒（1.03 ~ 1.16 g/ml）不同。这些结果表明感染性HCV颗粒在细胞内组装，并且在病毒输出过程中其生化组成发生改变。
The recent development of a cell culture infection model for hepatitis C virus (HCV) permits the production of infectious particles in vitro. In this report, we demonstrate that infectious particles are present both within the infected cells and in the supernatant. Kinetic analysis indicates that intracellular particles constitute precursors of the secreted infectious virus. Ultracentrifugation analyses indicate that intracellular infectious viral particles are similar in size (similar to 65 to 70 nm) but different in buoyant density (similar to 1.15 to 1.20 g/ml) from extracellular particles (similar to 1.03 to 1.16 g/ml). These results indicate that infectious HCV particles are assembled intracellularly and that their biochemical composition is altered during viral egress.