Smt3, a SUMO-1 homolog, is conjugated to Cdc3, a component of septin rings at the mother-bud neck in budding yeast.
Smt3, a SUMO-1 homolog, is conjugated to Cdc3, a component of septin rings at the mother-bud neck in budding yeast.
复制标题
Smt3 是 SUMO-1 同源物，与 Cdc3 缀合，Cdc3 是芽殖酵母母芽颈处隔膜环的一个组成部分。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
Yoshiko Kikuchi
中科院分区：
文献类型：
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作者：
Yoshimitsu Takahashi;Masayuki Iwase;Mineko Konishi;Masato Tanaka;Akio Toh;Yoshiko Kikuchi
酿酒酵母的SMT3基因是一个重要的基因，编码类似于哺乳动物SUMO-1的泛素样蛋白。当标记的Smt3或人类SUMO-1从GAL1启动子表达时，任一基因都挽救了Smt3干扰物的致命性。通过间接免疫荧光显微镜，ha标记的Smt3主要在细胞核中被检测到，也像septin纤维一样在母芽颈部被检测到。免疫沉淀实验表明，Smt3修饰了septin的组分之一Cdc3。此外，在限制温度下，ubc9-1突变体中Cdc3-Smt3偶联物的蛋白水平降低，septin环消失，Smt3偶联系统可能存在缺陷。因此，我们得出结论，Smt3在母芽颈部的间隔蛋白环中与Cdc3结合。在细胞分裂前后，Cdc3-Smt3偶联物消失。我们讨论了Smt3与septin组分结合的生物学意义。
SMT3 of Saccharomyces cerevisiae is an essential gene encoding a ubiquitin-like protein similar to mammalian SUMO-1. When a tagged Smt3 or human SUMO-1 was expressed from GAL1 promoter, either gene rescued the lethality of the smt3 disruptant. By indirect-immunofluorescent microscopy, the HA-tagged Smt3 was detected mostly in nuclei and also at the mother-bud neck just like septin fibers. Indeed immunoprecipitation experiments revealed that Cdc3, one of septin components, was modified with Smt3. Furthermore, the protein level of the Cdc3-Smt3 conjugate was reduced and the septin rings disappeared in a ubc9-1 mutant at a restrictive temperature, where the Smt3 conjugation system should be defective. Thus, we conclude that Smt3 was conjugated to Cdc3 in septin rings localized at the mother-bud neck. Around the time of cytokinesis the Cdc3-Smt3 conjugate disappeared. We discuss the biological significance of this Smt3 conjugation to a septin component.
影响因子：
3.3
作者：
MELUH, PB;KOSHLAND, D
通讯作者：
KOSHLAND, D
影响因子：
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作者：
J. Pringle;R. Preston;A. Adams;T. Stearns;D. Drubin;B. Haarer;E. Jones
通讯作者：
J. Pringle;R. Preston;A. Adams;T. Stearns;D. Drubin;B. Haarer;E. Jones