A NEW METHOD FOR SIMULTANEOUS PURIFICATION OF CYTOCHROME-B5 AND NADPH-CYTOCHROME-C REDUCTASE FROM RAT LIVER MICROSOMES
A NEW METHOD FOR SIMULTANEOUS PURIFICATION OF CYTOCHROME-B5 AND NADPH-CYTOCHROME-C REDUCTASE FROM RAT LIVER MICROSOMES
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DOI：
10.1093/oxfordjournals.jbchem.a129248
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发表时间：
1970-01-01
影响因子：
2.7
通讯作者：
TAKESUE, S
中科院分区：
文献类型：
--
作者：
OMURA, T;TAKESUE, S
本文报道了一种从大鼠肝微粒体中分离纯化细胞色素B_5和NADPH-细胞色素C还原酶的新方法。该程序是先前报道的小规模程序的准备应用，适合于从几百克或更多的大鼠肝脏开始同时提纯两种酶。该方法也适用于其他动物的肝微体，从微体中回收细胞色素b5和NADPH-细胞色素c还原酶的回收率为35-45%，并通过沉降法和圆盘电泳法证实了纯化制剂的均一性。纯化的NADPH-细胞色素c还原酶可被NADPH或NADH还原，并且具有很弱的NADH连接的还原酶活性。
A new procedure for the purification of cytochromeb5and NADPH-cytochrome c reductase from rat liver microsomes is described. This procedure is a preparative application of a small-scale procedure reported earlier, and is suitable for purifying both enzymes simultaneously starting from several hundred grams or more of rat liver. The method is applicable to liver microsomes of other animals.The recovery of cytochromeb5and NADPH-cytochrome c reductase from microsomes was 35–45%, and the homogeneity of purified preparations was confirmed by sedimentation analysis and disc electrophoresis. The high purity of the preparations obtained by the previous small-scale procedure was also confirmed.Purified NADPH-cytochrome c reductase was reducible by either NADPH or NADH, and had a very weak NADH-linked reductase activity.