Genome analysis of the Clostridium difficile phage ΦCD6356, a temperate phage of the Siphoviridae family
Genome analysis of the Clostridium difficile phage ΦCD6356, a temperate phage of the Siphoviridae family
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DOI：
10.1016/j.gene.2010.04.010
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发表时间：
2010-08-15
期刊：
影响因子：
3.5
通讯作者：
Ross, R. Paul
中科院分区：
文献类型：
--
作者：
Horgan, Marianne;O'Sullivan, Orla;Ross, R. Paul
对43株艰难梭菌进行丝裂霉素C诱导后，分离并鉴定了温带噬菌体Phi CD6356和Phi CD6365。这两个噬菌体都属于噬菌体病毒科家族，根据序列数据，Phi CD6356的基因组大小为37,664 bp，根据限制性内切酶分析，Phi CD6365的基因组大小约为50 kb。蛋白质分析显示类似的蛋白质图谱，并表明Phi CD6356主要衣壳蛋白的翻译后加工。Phi CD6356的基因组序列与以前报道的其他噬菌体序列有很大的不同，在59个预测的开放阅读框中，只有21个可能具有推测的功能。然而，基因组组织与感染低GC含量革兰氏阳性细菌的Siphopviridae家族其他成员非常相似。衣壳蛋白的模块化组织、基因组同步性、粘性末端的存在和翻译后加工表明Phi CD6356是拟议的Sfi21样属的成员。据我们所知，这份报告是第一个被测序的艰难梭菌噬菌体。(C)2010爱思唯尔B.V.保留所有权利。
The temperate phages Phi CD6356 and Phi CD6365 were isolated and characterised following mitomycin C induction of 43 Clostridium difficile strains. Both phages belong to the Siphoviridae family and have genome sizes of 37,664 bp for Phi CD6356 based on sequence data and approximately 50 kb for Phi CD6365 based on restriction analysis. Protein analysis revealed similar protein profiles and indicated posttranslational processing of the Phi CD6356 major capsid protein. The genome sequence of Phi CD6356 is substantially different from other previously reported phage sequences and a putative function could be assigned to only 21 out of 59 predicted open reading frames. However, the genome organisation closely resembles that of other members of the Siphoviridae family which infect low GC-content Gram-positive bacteria. The modular organisation, genome synteny, presence of cohesive ends and posttranslational processing of the capsid protein suggest Phi CD6356 is a member of the proposed Sfi21-like genera. To our knowledge, this report represents the first C difficile phage of the Siphoviridae family to be sequenced. (C) 2010 Elsevier B.V. All rights reserved.