Isolation and identification of entomopathogenic nematodes and their symbiotic bacteria from Herault and Gard (Southern France)
Isolation and identification of entomopathogenic nematodes and their symbiotic bacteria from Herault and Gard (Southern France)
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DOI：
10.1016/j.jip.2008.01.006
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发表时间：
2008-06-01
影响因子：
3.4
通讯作者：
Sicard, Mathieu
中科院分区：
文献类型：
--
作者：
Emelianoff, Vanya;Le Brun, Nathalie;Sicard, Mathieu
分离和鉴定本地线虫细菌协会在现场是必要的，在特定的位置，地方性害虫的成功控制。尚未进行研究，以恢复和确定EPN在法国大都市。在本文中，我们提供的EPN和他们的共生细菌在法国南部的埃罗和加尔地区进行的调查结果。分离的线虫的分子特征描绘了三个不同的斯氏线虫属物种和一个异小杆线虫属物种，H。细菌门。回收的斯氏线虫属物种被鉴定为：S. feltiae和S.仿射和未描述的物种。共生菌经鉴定为X. bovienii对S. feltiae和S.仿射系统发育分析表明，新的未描述的斯氏线虫属（Steinernema sp.）arenarium，但分歧足以假设它属于一个新的物种内的“glaseri-group”。该线虫的共生体为X. kozodoii。所有回收的异小杆线虫分离株均被诊断为H。细菌和共生菌鉴定为发光杆菌（Photorhabdusluminescens）。这些线虫的分子特征使得能够区分两种不同的H。细菌菌株。细菌共生，这两个H。细菌属菌株被鉴定为P. lurninescens ssp. kayaii和发光假单胞菌P. luminescens ssp.劳蒙地。(c)2008年爱思唯尔公司All rights reserved.
Isolation and identification of native nematode-bacterial associations in the field are necessary for successful control of endemic pests in a particular location. No study has yet been undertaken to recover and identify EPN in metropolitan France. In the present paper, we provide results of a survey of EPN and their symbiotic bacteria conducted in Herault and Gard regions in Southern France. Molecular characterization of isolated nematodes depicted three different Steinernema species and one Heterorhabditis species, H. bacteriophora. Steinernema species recovered were identified as: S. feltiae and S. affine and an undescribed species. Xenorhabdus symbionts were identified as X. bovienii for both S. feltiae and S. affine. Phylogenetic analysis placed the new undescribed Steinernema sp. as closely related to S. arenarium but divergent enough to postulate that it belongs to a new species within the "glaseri-group"., The Xenorhabdus symbiont from this Steinernema sp. was identified as X. kozodoii. All Heterorhabditis isolates recovered were diagnosed as H. bacteriophoro and their bacterial symbionts were identified as Photorhabdus luminescens. Molecular characterization of these nematodes enabled the distinction of two different H. bacteriophora strains. Bacterial symbiontic, strains of these two H. bacteriophora strains were identified as P. lurninescens ssp. kayaii and P. luminescens ssp. laumondii. (c) 2008 Elsevier Inc. All rights reserved.