The use of polylactic acid matrix and periosteal grafts for the reconstruction of rabbit knee articular defects.
The use of polylactic acid matrix and periosteal grafts for the reconstruction of rabbit knee articular defects.
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DOI：
10.1002/jbm.820250305
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发表时间：
1991-03
期刊：
影响因子：
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通讯作者：
Herbert P. von Schroeder;Michael K. Kwan;David Amiel;Richard D. Coutts
中科院分区：
文献类型：
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作者：
Herbert P. von Schroeder;Michael K. Kwan;David Amiel;Richard D. Coutts
为了寻找一种改善软骨修复的材料，我们研究了多孔聚乳酸基质（PLA）带骨膜和不带骨膜移植在18只新西兰大白兔膝关节股骨内侧髁大关节缺损中的应用。右膝缺损用聚乳酸填充，左膝缺损用聚乳酸和骨膜移植物填充。所有动物均于12周时处死。聚乳酸在所有标本的关节表面允许新软骨的新生生长，并似乎作为细胞迁移和基质形成的支架。组织学上，少量PLA保留在新软骨下，大部分被骨取代。PLA是骨膜移植的合适载体，具有较高的移植成活率（89%）和新软骨的增殖，新软骨比单独PLA膝关节更厚，更接近关节软骨。生物化学方面，移植膝关节中II型胶原含量（83%）高于单纯PLA膝关节（65%）。新软骨的生物力学测试包括平衡位移、骨料模量和表观渗透性。这些测试在单独PLA和移植膝关节之间没有统计学差异。与正常软骨相比，新软骨硬度较低，但通透性相似。采用骨膜移植和不采用骨膜移植均可获得关节缺损的一致修复，形成与透明关节软骨非常相似的组织。
In order to find a material that would improve cartilage repair, we investigated the use of porous polylactic acid matrix (PLA) with and without periosteal grafts in large articular defects in the medial femoral condyles of 18 New Zealand white rabbit knees. The right knee defect was filled with PLA, the left defect was filled with PLA and a periosteal graft. All animals were killed at 12 weeks. PLA allowed for the de novo growth of neocartilage at the articular surface in all specimens and appeared to serve as a scaffolding for cell migration and matrix formation. Histologically, small amounts of PLA remained under the neocartilage with the majority being replaced by bone. PLA was a suitable carrier for periosteal grafts with a high graft survival rate (89%) and proliferation of a neocartilage which was thicker and more closely resembled articular cartilage than PLA alone knees. Biochemically, there was more type II collagen in the grafted knees (83%) than in the PLA alone knees (65%). Biomechanical tests of the neocartilage included equilibrium displacement, aggregate modulus, and apparent permeability. These tests were not statistically different between PLA alone and grafted knees. Comparison to normal cartilage indicated that the neocartilage was less stiff but had similar permeability. A consistent repair of the articular defects was achieved with and without periosteal grafts resulting in a tissue that closely resembled hyaline articular cartilage.