Evaluation of emotional behaviors in young offspring of C57BL/6J mice after gestational and/or perinatal exposure to nicotine in six different time-windows
Evaluation of emotional behaviors in young offspring of C57BL/6J mice after gestational and/or perinatal exposure to nicotine in six different time-windows
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DOI：
10.1016/j.bbr.2012.10.058
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发表时间：
2013-02-15
影响因子：
2.7
通讯作者：
Nabeshima, Toshitaka
中科院分区：
文献类型：
--
作者：
Alkam, Tursun;Kim, Hyoung-Chun;Nabeshima, Toshitaka
在怀孕期间，尼古丁替代疗法正被用于辅助戒烟。然而，由于缺乏系统的研究，产前暴露的尼古丁对后代情绪行为的影响尚不清楚。因此，目前的研究最初旨在评估在六个不同的时间窗内经历妊娠期和/或围产期尼古丁暴露（GPNE）的幼鼠后代（出生后28-36天）的情绪行为。在妊娠第0 ~第13天（g0 ~ g13）、第14 ~围产期第0天（P0）、第0 ~ P0、第14 ~ p7、第0 ~ p7、第0 ~ p7天6个不同的时间窗口，通过加糖（2%蔗糖）的饮用水暴露于尼古丁。在p28 ~ p36天，对雄性和雌性后代进行一系列行为测试，包括明暗箱测试、弹珠掩埋行为测试、新奇性抑制进食测试、社交性和社会新奇性偏好测试、社交回避管测试和升高+迷宫测试。G0-P0、G14-P0、G14-P7、G0-P7期间GPNE对雌雄后代的异常行为有不同程度的诱导作用。结果表明，尼古丁在妊娠期和/或围产期的任何时间点都会损害后代的情绪行为，并为进一步研究gpne诱导的情绪异常的神经化学或分子关系提供了一定的时间窗口。(c) 2012 Elsevier B.V.版权所有
Nicotine replacement treatments are being alternatively applied as an aid to smoking cessation during pregnancy. However, the effects of nicotine exposed at the prenatal stage on the emotional behaviors in offspring are not well understood due to the lack of systematic investigations. The current study has therefore initially aimed to evaluate emotional behaviors in young mouse offspring (postnatal day 28-36) which experienced gestational and/or perinatal nicotine exposure (GPNE) in six different time-windows. Pregnant C57BL/6J mice were exposed to nicotine via sweetened (2% sucrose) drinking water during 6 different time-windows including gestational day 0-day 13 (G0-G13), G14-perinatal day 0 (P0), G0-P0, G14-P7, G0-P7, and P0-P7. During P28-P36 days, both male and female offspring were given a battery of behavioral tests including light and dark box test, marble burying behavior test, novelty-suppressed feeding test, sociability and social novelty preference test, social avoidance tube test, and elevated plus maze test. GPNE during G0-P0, G14-P0, G14-P7, and G0-P7 induced abnormal behaviors in male and female offspring to different extent. Results indicated that nicotine at any time points of gestational and/or perinatal period impairs emotional behaviors in offspring, and suggested certain time-windows for further neurochemical or molecular studies in relation with GPNE-induced emotional abnormalities. (c) 2012 Elsevier B.V. All rights reserved.