Cerebral ischemia upregulates vascular endothelin ETB receptors in rat
Cerebral ischemia upregulates vascular endothelin ETB receptors in rat
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DOI：
10.1161/01.str.0000028183.04277.32
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发表时间：
2002-09-01
期刊：
影响因子：
8.3
通讯作者：
Edvinsson, L
中科院分区：
文献类型：
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作者：
Stenman, E;Malmsjo, M;Edvinsson, L
背景和目的：内皮素1（ET 1）在脑缺血中水平升高，如果血管受体改变，ET 1的作用可能更重要。我们探讨了脑缺血中ET受体表达是否有任何变化。用血管内细丝法阻断雄性Wistar大鼠的基底动脉2小时，再循环46小时后切除基底动脉和双侧MCA。结果：对新鲜或假手术大鼠脑动脉无收缩作用的Sarafotoxin 6c（S6 c）可引起闭塞MCA的显著收缩（E-m [1]）。最大收缩量占63.5 mmol/L K+的收缩量的百分比=68+/-68%P
Background and Purpose-Elevated levels of endothelin 1 (ET 1) have been reported in cerebral ischemia A role for ET may prove more important if the vascular receptors were changed We addressed whether there is any change in ET receptor expression in cerebral ischemiaMethods-The right middle cerebral artery (MCA) was occluded in male Wistar rats for 2 hours with the intraluminal filament method The basilar artery and both MCAs were removed after 46 hours of recirculation The contractile responses to ET 1 a combined ETA and ETB receptor agonist and sarafotoxin 6c (S6c) a selective ETB receptor agonist were examined in vitro and ET receptor mRNA was quantified by real time polymerase chain reactionResults-S6c which had no contractile effect per se on fresh or sham operated rat cerebral arteries induced a marked contraction in the occluded MCA (E-m [maximum contraction calculated as percentage of the contractile capacity of 63 5 mmol/L K+] =68+/-68% P