Common and distinct gene expression patterns induced by the herbicides 2,4-dichlorophenoxyacetic acid, cinidonethyl and tribenuron-methyl in wheat
Common and distinct gene expression patterns induced by the herbicides 2,4-dichlorophenoxyacetic acid, cinidonethyl and tribenuron-methyl in wheat
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DOI：
10.1002/ps.1291
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发表时间：
2006-12-01
影响因子：
4.1
通讯作者：
Keller, Beat
中科院分区：
文献类型：
--
作者：
Pasquer, Frederique;Ochsner, Urs;Keller, Beat
在小麦中，除草剂用于控制杂草。对除草剂处理后耐受植物的代谢变化知之甚少。利用cDNA微阵列技术研究了2，4-二氯苯氧乙酸（2，4-D）、西尼酮和苯磺隆对小麦转录组的影响。在处理后24小时和2周之间研究了在受控环境中或在田间生长的植物的基因表达。在受控条件下，2，4-D在处理后不久诱导苯丙素途径的基因。Cinidon-ethyl触发过氧化物酶和防御相关的基因表达在受控条件下，可能是因为活性氧释放的光氧化的原卟啉-IX。在受控条件下，相同的基因在田间被上调，尽管水平较弱。这些结果表明，cinidon-ethyl特异性诱导参与植物防御的基因。在受控条件下，苯磺隆处理后没有立即改变表达谱，但1周后，防御相关基因上调。磺酰脲类化合物如苯磺隆特异性抑制乙酰乳酸合成酶，并迅速解毒，但某些代谢产物的活性可以解释基因表达的后期变化。最后，在磺酰脲处理后，在田间观察到参与支链氨基酸合成的异丙苹果酸合酶基因和防御相关基因的过度表达。(c)2006年化学工业协会
In wheat, herbicides are used to control weeds. Little is known about the changes induced in the metabolism of tolerant plants after herbicide treatment. The impact of three herbicides [2,4-dichlorophenoxyacetic acid (2,4-D), cinidon-ethyl and tribenuron-methyl] on the wheat transcriptome was studied using cDNA microarrays. Gene expression of plants grown in a controlled environment or in the field was studied between 24 h and 2 weeks after treatment. Under controlled conditions, 2,4-D induced genes of the phenylpropanoid pathway soon after treatment. Cinidon-ethyl triggered peroxidase and defence-related gene expression under controlled conditions, probably because reactive oxygen species are released by photo-oxidation of protoporphyrin-IX. The same genes were upregulated in the field as under controlled conditions, albeit at a weaker level. These results show that cinidon-ethyl specifically induces genes involved in plant defence. Under controlled conditions, tribenuron-methyl did not change the expression profile immediately after treatment, but defence-related genes were upregulated after 1 week. Sulfonylurea compounds such as tribenuron-methyl specifically inhibit acetolactate synthase and are rapidly detoxified, but the activity of some of the resulting metabolites could explain later changes in gene expression. Finally, overexpression of the isopropylmalate synthase gene, involved in branched-chain amino acid synthesis, and of defence-related genes was observed in the field after sulfonylurea treatment. (c) 2006 Society of Chemical Industry