Development of tobacco smoke-induced lung tumors in mice fed Bowman-Birk protease inhibitor concentrate (BBIC).
Development of tobacco smoke-induced lung tumors in mice fed Bowman-Birk protease inhibitor concentrate (BBIC).
复制标题
喂食 Bowman-Birk 蛋白酶抑制剂浓缩物 (BBIC) 的小鼠中烟草烟雾诱发的肺部肿瘤的发生。
DOI：
10.1016/s0304-3835(02)00156-8
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发表时间：
2002
期刊：
影响因子：
9.7
通讯作者：
Espiritu,Imelda
中科院分区：
文献类型：
--
作者：
Witschi,Hanspeter;Espiritu,Imelda
雄性 A/J 品系小鼠每天 6 小时、每周 5 天暴露于 89% 香烟侧流烟雾和 11% 香烟主流烟雾的混合物中，持续 5 个月，然后在空气中再恢复 4 个月。在烟雾暴露期间、烟雾暴露后或整个 9 个月期间，给动物喂食浓度为 AIN-93G 饮食中 1% 的 Bowman-Birk 蛋白酶抑制剂浓缩物 (BBIC)。实验结束时，确定肺部肿瘤的发生率和复数性。在阳性对照实验中，A/J品系小鼠被注射3-甲基胆蒽（MCA）并饲喂含有1%BBIC的饮食； 5个月后，这些动物被杀死。研究发现，在接受 MCA 治疗的动物中，BBIC 降低了肺肿瘤的多重性，而在暴露于烟雾的小鼠中，BBIC 并没有调节肺肿瘤的发展。
Male strain A/J mice were exposed, 6h a day, 5 days a week for 5 months to a mixture of 89% cigarette sidestream and 11% cigarette mainstream smoke and then allowed to recover for another 4 months in air. The animals were fed Bowman-Birk protease inhibitor concentrate (BBIC) at a concentration of 1% in AIN-93G diet either during smoke exposure, following smoke exposure or during the entire 9 months. At the end of the experiment, the incidence and multiplicity of lung tumors were determined. In a positive control experiment, strain A/J mice were injected with 3-methylcholanthrene (MCA) and fed a diet containing 1% BBIC; these animals were killed 5 months later. It was found that in the animals treated with MCA, BBIC decreased lung tumor multiplicities, whereas in the smoke exposed mice, BBIC did not modulate lung tumor development.