Transplantation of adipose derived stromal cells is associated with functional improvement in a rat model of chronic myocardial infarction
Transplantation of adipose derived stromal cells is associated with functional improvement in a rat model of chronic myocardial infarction
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DOI：
10.1016/j.ejheart.2008.03.017
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发表时间：
2008-05-01
影响因子：
18.2
通讯作者：
Prosper, Felipe
中科院分区：
文献类型：
--
作者：
Mazo, Manuel;Planat-Benard, Valerie;Prosper, Felipe
目的：确定在慢性心肌梗死模型中，与骨髓单核细胞（BM-MNC）相比，未分化和心脏预分化脂肪干细胞移植的效果。方法：对 95 只 Sprague-Dawley 大鼠进行左冠状动脉结扎，1 个月后直接心肌内注射脂肪干细胞 (ADSC)、心肌细胞 (AD-CMG) 或来自增强型绿色荧光蛋白 (eGFP) 小鼠的 BM-MNC。对照组用培养基处理。通过超声心动图和 F-18-FDG microPET 评估心脏功能。还通过（免疫）组织化学和免疫荧光评估了疤痕组织中的细胞植入、分化、血管生成和纤维化。结果：细胞移植后一个月，ADSC 诱导心脏功能显着改善（LVEF 46.3 +/- 9.6% 对比移植前的 27.7 +/- 8%）和组织活力（64.78 +/- 7.2%） 对比移植前的 55.89 +/- 6.3%）。还检测到血管生成程度的增加和纤维化的减少。尽管 AD-CMG 或 BM-MNC 移植对梗塞心脏的血管生成和纤维化也有积极的影响（尽管较小），但这种益处并没有转化为心脏功能或组织活力的显着改善。结论：这些结果表明，在慢性梗塞中移植脂肪来源的细胞比心脏预分化的 ADSC 和 BM-MNC 具有更好的益处。 (C) 2008 年欧洲心脏病学会。由 Elsevier B.V. 出版。保留所有权利。
Aims: To determine the effect of transplantation of undifferentiated and cardiac pre-differentiated adipose stem cells compared with bone marrow mononuclear cells (BM-MNC) in a chronic model of myocardial infarction.Methods: Ninety-five Sprague-Dawley rats underwent left coronary artery ligation and after I month received by direct intramyocardial injection either adipose derived stem cells (ADSC), cardiomyogenic cells (AD-CMG) or BM-MNC from enhanced-Green Fluorescent Protein (eGFP) mice. The control group was treated with culture medium. Heart function was assessed by echocardiography and F-18-FDG microPET. Cell engraftment, differentiation, angiogenesis and fibrosis in the scar tissue were also evaluated by (immuno)histochemistry and immunofluorescence.Results: One month after cell transplantation, ADSC induced a significant improvement in heart function (LVEF 46.3 +/- 9.6% versus 27.7 +/- 8% pre-transplant) and tissue viability (64.78 +/- 7.2% versus 55.89 +/- 6.3% pre-transplant). An increase in the degree of angiogenesis and a decrease in fibrosis were also detected. Although transplantation of AD-CMG or BM-MNC also had a positive, albeit smaller, effect on angiogenesis and fibrosis in the infarcted hearts, this benefit did not translate into a significant improvement in heart function or tissue viability.Conclusion: These results indicate that transplantation of adipose derived cells in chronic infarct provides a superior benefit to cardiac pre-differentiated ADSC and BM-MNC. (C) 2008 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.