A novel filterless fluorescence detection sensor for DNA analysis
A novel filterless fluorescence detection sensor for DNA analysis
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DOI：
10.1109/ted.2005.864385
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发表时间：
2006-03-01
影响因子：
3.1
通讯作者：
Ishida, M
中科院分区：
文献类型：
--
作者：
Maruyama, Y;Sawada, K;Ishida, M
提出了一种片上光谱学的新方法，该方法无需带通滤波器或光栅即可运行。光谱学原理基于光吸收系数随波长的差异。光强是根据在硅中的不同穿透深度计算得出的。这种新型光谱仪的关键在于使用了光电门有源像素，它利用栅极电压对光生电荷进行选择性控制。为了演示这种光谱仪，我们实验室利用标准的5微米CMOS硅集成电路技术制造了一种新型无滤光片荧光检测传感器。当受到470纳米辐射激发时，SYBR - Green标记在520纳米处发出荧光。在使用两个发光二极管的模拟实验中，检测到的荧光强度是激发光强度的1/300（荧光强度为1微瓦/平方厘米，而激发光强度为300微瓦/平方厘米）。在使用含有SYBR - Green的实际DNA溶液的实验中，证实了荧光检测传感器无需滤光片即可成功检测到荧光标记。
A new method is presented of on-chip optical spectroscopy, functioning without the need for a bandpass filter or grating. The principle of optical spectroscopy is based on the difference of optical absorption coefficients with wavelength. The optical intensity is calculated from the different penetration depths in Si. The key to the new spectrometer is the use of a photogate active pixel providing the selective control of photo-generated charge using the gate voltage. To demonstrate this spectrometer, a novel filterless fluorescence detection sensor has been fabricated in our laboratory, using standard 5-mu m CMOS silicon integrated circuit technology. The SYBR-Green label fluoresces at 520 nm when exited by 470-nm radiation. In a simulation experiment using two LEDs, the fluorescent intensity detected was 1/300 of the excitation light intensity (intensity of fluorescence was 1 mu W/cm(2), while the excitation illumination was 300 mu W/cm(2)). In an experiment using actual DNA solution containing SYBR-Green, it was confirmed that the fluorescence detection sensor successfully detected the fluorescent label without the need for a filter.