Identification of expressed sequence tags (ESTs) of the highly transcribed genes in Aspergillus niger

Identification of expressed sequence tags (ESTs) of the highly transcribed genes in Aspergillus niger
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DOI:
10.1023/a:1005439205957
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发表时间:
1999-05-01
影响因子:
2.7
通讯作者:
Chae, KS
Chae, KS
中科院分区:
工程技术4区
文献类型:
--
作者:
Choi, JY;Lee, DW;Chae, KS

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确定黑曲霉 3' 定向 cDNA 文库中 285 个随机选择的克隆的序列,可以识别高表达基因的表达序列标签 (EST)。 1个EST出现了7次,1个出现了6次,1个出现了5次,4个出现了3次,12出现了两次。在这19个EST中,GenBank中鉴定出了10个,但没有一个是黑曲霉的,这表明黑曲霉中存在大量高表达的未鉴定基因。
Determined sequences of 285 randomly selected clones in a 3'-directed cDNA library of Aspergillus niger could identify expressed seqeunce tags (ESTs) of genes highly expressed. One EST appeared seven times, one six times, one five times, four three times and 12 twice. Out of these 19 ESTs, ten were identified in GenBank, but none was of A. niger, suggesting that there are a lot of unidentified genes highly expressed in A. niger.