Inhibition of rabbit neutrophil lysosomal enzyme secretion, non-stimulated and chemotactic factor stimulated locomotion by nordihydroguaiaretic acid.
Inhibition of rabbit neutrophil lysosomal enzyme secretion, non-stimulated and chemotactic factor stimulated locomotion by nordihydroguaiaretic acid.
复制标题
去甲二氢愈创木酸抑制兔中性粒细胞溶酶体酶分泌、非刺激和趋化因子刺激运动。
DOI：
10.1016/0024-3205(80)90191-5
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发表时间：
1980
期刊：
影响因子：
6.1
通讯作者：
Elmer L. Becker
中科院分区：
文献类型：
--
作者：
H. Showell;P. Naccache;R. Sha’afi;Elmer L. Becker
去甲二氢愈创木酸在细胞松弛素B存在下不可逆地抑制由趋化因子甲硫氨酰亮氨酰苯丙氨酸和C5 a诱导的兔腹膜中性粒细胞的Ca++依赖性和非依赖性溶酶体酶释放。抑制作用具有浓度和时间依赖性。此外，花生四烯酸和Ca++载体A23187诱导的细胞松弛素B依赖性释放也受到类似的抑制。类似浓度的NDGA也抑制中性粒细胞运动和趋化因子增强的运动，如使用改良的Boyden室所测量的。由于去甲二氢愈创木酸已被证明是脂氧合酶活性的抑制剂，因此花生四烯酸代谢的这一途径可能在中性粒细胞运动和促分泌素诱导的细胞松弛素B依赖性溶酶体酶释放中很重要。
Nordihydroguaiaretic acid irreversibly inhibits both Ca++dependent and independent lysosomal enzyme release from rabbit peritoneal neutrophils induced by the chemotactic factors, formyl-methionyl-leucyl-phenylalanine and C5a in the presence of cytochalasin B. The inhibition is both concentration and time dependent. In addition, the cytochalasin B dependent release induced by arachidonic acid and the Ca++ionophore A23187 is similarly inhibited. Similar concentrations of NDGA also inhibit neutrophil locomotion and chemotactic factor enhanced locomotion, as measured using modified Boyden chambers. As nordihydroguaiaretic acid has been shown to be an inhibitor of lipoxygenase activity, it is possible that this pathway of arachidonic acid metabolism is important in neutrophil locomotion and in cytochalasin B dependent lysosomal enzyme release induced by secretagogues.