Insulin action, regional fat, and myocyte lipid: Altered relationships with increased adiposity
Insulin action, regional fat, and myocyte lipid: Altered relationships with increased adiposity
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DOI：
10.1038/oby.2003.176
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发表时间：
2003-11-01
期刊：
影响因子：
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通讯作者：
Chisholm, DJ
中科院分区：
文献类型：
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作者：
Gan, SK;Kriketos, AD;Chisholm, DJ
目的：腹部脂肪和肌细胞甘油三酯水平与胰岛素敏感性呈负相关，但在超重中，它们的相互关系尚未充分表征。使用最新的测量肌细胞内甘油三酯的方法，在患有广泛肥胖的男性中研究了这些关系。研究方法和程序：肌细胞甘油三酯含量（比目鱼肌和胫骨前肌的 H-1 磁共振波谱和股外侧肌活检的生化评估）、区域脂肪分布（DXA 和腹部磁共振成像）、血脂、胰岛素作用（正常血糖高胰岛素钳夹）和底物氧化率（间接）对 39 名患有广泛 2 肥胖（BMI 28.6 +/- 4.1 kg/m(2)，范围 20.1 至 37.6 kg/m(2)）的非糖尿病男性（35.1 +/- 7.8 岁）进行了测量。结果：胰岛素刺激的葡萄糖处理与局部身体脂肪库之间的关系似乎用非线性模型比线性模型更合适地描述。当使用中位全身脂肪作为切点对组进行细分时，在正常/超重程度较低的情况下，胰岛素刺激的葡萄糖处理与所有区域身体脂肪测量值（所有 p 小于或等于 0.004）、血清甘油三酯和游离脂肪酸（p < 0.02）以及比目鱼肌肌细胞内脂质（p = 0.003）和股外侧肌甘油三酯（p = 0.04）呈负相关。组。相比之下，在超重男性中，只有内脏腹部脂肪与胰岛素刺激的葡萄糖处理呈显着负相关（r = -0.576，p = 0.01），其中一些人的肌细胞脂质水平令人惊讶地低于预期。当使用不同的肥胖切点或测量方法对组进行细分时，这些发现仍然存在。讨论：随着肥胖的增加，身体脂肪库、肌细胞甘油三酯、血脂和胰岛素作用之间的相互关系通常不存在。然而，内脏腹部脂肪与总肥胖的关系不太密切，但对于正常肥胖和肥胖增加的男性来说，它仍然是胰岛素抵抗的重要预测因子。
Objective: Abdominal fat and myocyte triglyceride levels relate negatively to insulin sensitivity, but their interrelationships are inadequately characterized in the overweight. Using recent methods for measuring intramyocyte triglyceride, these relationships were studied in men with a broad range of adiposity.Research Methods and Procedures: Myocyte triglyceride content (H-1-magnetic resonance spectroscopy of soleus and tibialis anterior muscles and biochemical assessment of vastus lateralis biopsies), regional fat distribution (DXA and abdominal magnetic resonance imaging), serum lipids, insulin action (euglycemic hyperinsulinemic clamp), and substrate oxidation rates (indirect calorimetry) were measured in 39 nondiabetic men (35.1 +/- 7.8 years) with a broad range 2 of adiposity (BMI 28.6 +/- 4.1 kg/m(2), range 20.1 to 37.6 kg/m(2)).Results: Relationships between insulin-stimulated glucose disposal and regional body fat depots appeared more appropriately described by nonlinear than linear models. When the group was subdivided using median total body fat as the cut-point, insulin-stimulated glucose disposal correlated negatively to all regional body fat measures (all p less than or equal to 0.004), serum triglycerides and free fatty acids (p < 0.02), and both soleus intramyocellular lipid (p = 0.003) and vastus lateralis triglyceride (p = 0.04) in the normal/less overweight group. In contrast, only visceral abdominal fat showed significant negative correlation with insulin-stimulated glucose disposal in more overweight men (r = -0.576, p = 0.01), some of whom surprisingly had lower than expected myocyte lipid levels. These findings persisted when the group was subdivided using different cut-points or measures of adiposity.Discussion: Interrelationships among body fat depots, myocyte triglyceride, serum lipids, and insulin action are generally absent with increased adiposity. However, visceral abdominal fat, which corresponds less closely to total adiposity, remains an important predictor of insulin resistance in men with both normal and increased adiposity.