Biolistic Approach for Transient Gene Expression Studies in Plants
Biolistic Approach for Transient Gene Expression Studies in Plants
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DOI：
10.1007/978-1-0716-0356-7_6
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Citovsky, Vitaly
中科院分区：
文献类型：
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作者：
Lacroix, Benoi T.;Citovsky, Vitaly
自20世纪80年代末问世以来，通过高速微射弹（biolistic或微粒轰击）将外源核酸递送到活细胞中一直是农业和基础植物研究的宝贵工具。在这里，我们回顾的技术方面和主要应用的生物枪方法的研究涉及植物细胞中的瞬时基因表达。这些研究涵盖了植物研究的多个领域，包括基因表达、蛋白质亚细胞定位和细胞间运动、植物病毒学、沉默以及最近开发的通过瞬时表达定制核酸内切酶的靶向基因组编辑。
Since its inception in the late 1980s, the delivery of exogenous nucleic acids into living cells via high-velocity microprojectiles (biolistic, or microparticle bombardment) has been an invaluable tool for both agricultural and fundamental plant research. Here, we review the technical aspects and the major applications of the biolistic method for studies involving transient gene expression in plant cells. These studies cover multiple areas of plant research, including gene expression, protein subcellular localization and cell-to-cell movement, plant virology, silencing, and the more recently developed targeted genome editing via transient expression of customized endonucleases.