High-Performance Molecular Imaging with MALDI Trapped Ion-Mobility Time-of-Flight (timsTOF) Mass Spectrometry
High-Performance Molecular Imaging with MALDI Trapped Ion-Mobility Time-of-Flight (timsTOF) Mass Spectrometry
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DOI：
10.1021/acs.analchem.9b03612
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发表时间：
2019-11-19
影响因子：
7.4
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
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作者：
Spraggins, Jeffrey M.;Djambazova, Katerina V.;Caprioli, Richard M.
成像质谱学(IMS)能够在细胞分辨率下对生物组织进行空间定向的分子评估。新的发展和技术对于揭示本土生理功能和疾病的分子驱动因素至关重要。仪器必须最大限度地提高空间分辨率、吞吐量、灵敏度和特异性，因为组织成像实验由数千到数百万个像素组成。在此，我们报道了一种基质辅助激光解吸电离(MALDI)俘获离子迁移率光谱(TIMS)成像平台的开发和应用。这台MALDI timsTOF样机具有10微米的空间分辨率和20像素/S吞吐量的分子成像能力。MALDI源使用Bruker SmartBeam 3-D激光系统，该系统可以产生正方形烧录图案
Imaging mass spectrometry (IMS) enables the spatially targeted molecular assessment of biological tissues at cellular resolutions. New developments and technologies are essential for uncovering the molecular drivers of native physiological function and disease. Instrumentation must maximize spatial resolution, throughput, sensitivity, and specificity, because tissue imaging experiments consist of thousands to millions of pixels. Here, we report the development and application of a matrix-assisted laser desorption/ionization (MALDI) trapped ion-mobility spectrometry (TIMS) imaging platform. This prototype MALDI timsTOF instrument is capable of 10 mu m spatial resolutions and 20 pixels/s throughput molecular imaging. The MALDI source utilizes a Bruker SmartBeam 3-D laser system that can generate a square burn pattern of