Fabrication of hybrid nanocapsules by calcium phosphate mineralization of shell cross-linked polymer micelles and nanocages
Fabrication of hybrid nanocapsules by calcium phosphate mineralization of shell cross-linked polymer micelles and nanocages
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DOI：
10.1021/nl050817w
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发表时间：
2005-07-01
期刊：
影响因子：
10.8
通讯作者：
Mann, S
中科院分区：
文献类型：
--
作者：
Perkin, KK;Turner, JL;Mann, S
以自组装壳层交联聚（丙烯酸-b-异戊二烯）（PAA（78）-b-PI 97）胶束或交联PAA纳米笼为模板，制备了新型聚合物-无机纳米笼。混合纳米结构的直径通常为50-70纳米，由封闭在连续10-20纳米厚的无定形磷酸钙表面层内的球形聚合物纳米颗粒或纳米笼组成。通过低过饱和度水平和通过在加入HPO 42-之前在富含羧酸根的PAA域内螯合Ca 2+离子，促进了磷酸钙与聚合物纳米颗粒特异性结合的成核。离子浓度的修改被用来控制磷酸钙表面层的厚度，并制备矿化交联PAA-b-PI胶束与可变壳渗透性。对于封闭在10或20 nm厚的磷酸钙层内的混合纳米结构，β-胡萝卜素进入矿化壳交联PAA-b-PI胶束的疏水PI核心的渗透性分别降低约50或100%。我们的研究结果表明，磷酸钙-聚合物交联纳米胶囊可能具有潜在的应用，如药物输送，生物成像和治疗的应用中使用的pH响应的生物相容性混合纳米结构。
Self-assembled shell cross-linked poly(acrylic acid-b-isoprene) (PAA(78)-b-PI97) micelles or cross-linked PAA nanocages in aqueous solution were used as templates for the preparation of novel polymer-inorganic nanocapsules. The hybrid nanostructures were typically 50-70 nm in diameter and consisted of spherical polymer nanoparticles or nanocages enclosed within a continuous 10-20 nm thick surface layer of amorphous calcium phosphate. Nucleation of calcium phosphate specifically in association with the polymer nanoparticles was facilitated by low supersaturation levels and by sequestration of Ca2+ ions within the carboxylate-rich PAA domains prior to addition of HPO42-. Modifications in ionic concentrations were used to control the calcium phosphate surface layer thickness and prepare mineralized cross-linked PAA-b-PI micelles with variable shell permeability. The permeability of beta-carotene into the hydrophobic PI core of mineralized shell cross-linked PAA-b-PI micelles was reduced by approximately 50 or 100% respectively for hybrid nanostructures enclosed within 10 or 20 nm thick calcium phosphate layers. Our results suggest that calcium phosphate-polymer cross-linked nanocapsules could have potential applications as pH-responsive biocompatible hybrid nanostructures for use in applications such as drug delivery, bioimaging, and therapeutics.