IMMUNOHISTOCHEMICAL LOCALIZATION OF THE ANDROGEN RECEPTOR IN RAT AND HUMAN TISSUES
IMMUNOHISTOCHEMICAL LOCALIZATION OF THE ANDROGEN RECEPTOR IN RAT AND HUMAN TISSUES
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DOI：
10.1210/endo-127-6-3180
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发表时间：
1990-12-01
期刊：
影响因子：
4.8
通讯作者：
WILSON, EM
中科院分区：
文献类型：
--
作者：
SAR, M;LUBAHN, DB;WILSON, EM
对大鼠生殖组织、颌下腺、垂体、脑和人前列腺中的雄激素受体（AR）进行免疫组化定位。利用两种多克隆抗体中的任何一种，对来自大鼠和人AR的序列的肽产生抗体。8 .mu。m，在异戊烷中冷冻，在多聚甲醛中固定，在亲和素-生物素过氧化物酶程序中使用免疫血清、免疫前血清和肽吸附免疫血清的免疫球蛋白G部分进行染色。正常大鼠的附睾、前列腺腹侧、精囊和退管腺泡上皮细胞核中可见明显的AR。去势后3天停用雄激素，导致受体免疫染色丧失，雄激素给药后15分钟内恢复。正常大鼠前列腺腹侧基质细胞染色不明显或较弱，而附睾基质细胞染色较明显。AR局限于睾丸间质室内和周围的细胞核，包括支持细胞、耻骨周围肌样细胞和间质细胞，在生殖细胞中检测不到。上颌下腺上皮细胞和大鼠垂体前叶细胞群显示出强烈的AR核染色。在大鼠大脑中，AR存在于下丘脑内侧视前核、弓形核和腹内侧核、杏仁核内侧核、CA-1海马和皮质。AR在人良性前列腺增生的腺泡上皮细胞中表现突出，在纤维肌肉良性增生的间质中也存在。前列腺腺癌间质和上皮细胞呈异质染色。这些研究结果表明，使用抗多肽多克隆抗体可以在多种组织和细胞类型中免疫组织化学检测AR。组织中AR的存在与其已知的雄激素反应性相关。
Immunohistochemical localization of the androgen receptor (AR) was performed in reproductive tissues, submaxillary gland, pituitary, and brain of the rat and in human prostate. AR was visualized using either of two polyclonal antibodies raised against peptides with sequences derived from rat and human AR. Tissue sections of 6.sbd.8 .mu.m, frozen in isopentane and fixed in paraformaldehyde, were stained using immunoglobulin G fractions of immune, preimmune, and peptide-adsorbed immune sera in the avidin-biotin peroxidase procedure. AR was prominent in nuclei of acinar epithelial cells of epididymis, ventral prostate, seminal vesicle, and ductus deferens from the intact rat. Androgen withdrawal, 3 days after castration, resulted in the loss of receptor immunostaining, which was restored within 15 min of androgen administration. Stromal cell staining was absent or weak in the ventral prostate of intact rats, but was more evident in the epididymis. AR was confined to nuclei of cells within and bordering the interstitial compartment of the testis, including Sertoli cells, pertibular myoid cells, and interstitial cells, and was undetectable in germ cells. Submaxillary gland epithelial cells and a population of rat anterior pituitary cells showed strong nuclear staining of AR. In rat brain, AR was present in the medial preoptic, arcurate, and ventromedial nuclei of the hypothalamus, the medial nucleus of the amygdala, the CA-1 hippocampus, and the cortex. AR was prominent in acinar epithelial cells in human benign prostatic hyperplasia and was also present in stroma of fibromuscular benign hyperplasia. Heterogeneous staining was observed in stromal and epithelial cells of prostatic adenocarcinoma. The results of these studies indicate that AR can be detected immunohistochemically in variety of tissues and cell types using antipeptide polyclonal antibodies. The presence of AR in tissues correlated with their known androgen responsiveness.