Cell biological effects of mechanical stimulations generated by focused extracorporeal shock wave applications on cultured human bone marrow stromal cells
Cell biological effects of mechanical stimulations generated by focused extracorporeal shock wave applications on cultured human bone marrow stromal cells
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DOI：
10.1016/j.scr.2013.05.010
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发表时间：
2013-09-01
影响因子：
1.2
通讯作者：
Bloch, Wilhelm
中科院分区：
文献类型：
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作者：
Suhr, Frank;Delhasse, Yvonne;Bloch, Wilhelm
人骨髓基质细胞(HBMSCs)因其在移植环境中的免疫调节特性和对组织再生的贡献而具有巨大的临床潜力。事实上，它们是治疗应用中最有前途的干细胞类型之一，也是密集研究的主题。然而，hBMSCs的临床应用一直受到其可用性的限制；它们是稀缺的细胞，分离和纯化起来很麻烦。此外，在再生实验中，它们很难靶向损伤部位。为了克服这些局限性，聚焦体外冲击波(fESW，0.2/0.3mJ*mm(-2))被应用于纯化的培养的hBMSCs。FESW(0.2mJ*mm(-2))刺激可增加hBMSCs的生长速度(p<；0.05)、增殖(p<；0.05)、迁移、细胞跟踪和伤口愈合(p<；0.05)，并降低细胞凋亡率(p<；0.05)。HBMSC迁移行为的增加是由肌动蛋白细胞骨架的主动重塑介导的，如定向应力纤维形成的增加(p<；0.05)：此外，fESW处理后hBMSCs保持其分化潜力，而0.2mJ*mm(-2)是最有效的应用。综上所述，我们的结果首次确立了hBMSCs的行为可以根据特定的机械刺激进行修改和优化。这些发现似乎特别有希望，因为它们表明机械应力预适应hBMSCs在没有遗传操作的情况下改善治疗性能，并且机械预适应的hBMSCs将有利于基于hBMSC的组织再生。因此，这种方法为在再生医学中充分挖掘这些细胞的潜力打开了大门。(C)2013爱思唯尔B.V.保留所有权利。
Human bone marrow stromal cells (hBMSCs) bear tremendous clinical potential due to their immunomodulatory properties in transplantation settings and their contribution to tissue regeneration. In fact, they are among the most promising types of stem-like cells for therapeutic applications and are the subject of intense research. However, the clinical use of hBMSCs has been confounded by limitations in their availability; they are scarce cells cumbersome to isolate and purify. Additionally, they are difficult to target to the site of injury in regeneration experiments. In order to combat these limitations, focused extracorporeal shock waves (fESW, 0.2/0.3 mJ * mm(-2)) were applied to purified, cultured hBMSCs. fESW (0.2 mJ * mm(-2)) stimulations were found to increase hBMSCs' growth rate (p < 0.05), proliferation (p < 0.05), migration, cell tracking and wound healing (p < 0.05, respectively), as well as to reduce the rate of apoptosis activation (p < 0.05). The increase in hBMSC migration behavior was found to be mediated by active remodeling of the actin cytoskeleton as indicated by increased directed stress fiber formations (p < 0.05): Furthermore, hBMSCs maintain their differentiation potentials after fESW treatment, whereas 0.2 mJ * mm(-2) is the most effective application. In conclusion, our results establish first-timely that hBMSCs behavior can be modified and optimized in response to defined mechanical stimulation. These findings appear particularly promising as they suggest that mechanical stress preconditions hBMSCs for improved therapeutic performance without genetic manipulations and that mechanically preconditioned hBMSCs will be advantageous for hBMSC-based tissue regeneration. Therefore, this approach opens the door for exploiting the full potential of these cells in regenerative medicine. (C) 2013 Elsevier B.V. All rights reserved.