Quantification of presenilin-1 mRNA in Alzheimer's disease brains
Quantification of presenilin-1 mRNA in Alzheimer's disease brains
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DOI：
10.1016/0014-5793(96)00969-6
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发表时间：
1996-10-07
期刊：
影响因子：
3.5
通讯作者：
Cowburn, RF
中科院分区：
文献类型：
--
作者：
Johnston, JA;Froelich, S;Cowburn, RF
早老素-1（PS-1）基因位于14号染色体上，与早发性家族性阿尔茨海默病（AD）共分离。我们采用溶液杂交-RNase保护法检测了14例AD患者、9例非痴呆对照者和5例其他神经系统疾病患者死后颞中皮质和上级额叶皮质中PS-1 mRNA的表达。APP和APLP 2 mRNA先前已经在这些样品中定量（约翰斯顿等人，（1996）Mel. Brain Res.，阿尔茨海默病患者颞叶中部或上级额叶皮质中PS-1 mRNA水平与对照组相比无显著差异，PS-1 mRNA水平相当于总APP的10%和APLP 2 mRNA水平的30%，且不受年龄、死后延迟、组织pH值、在APOE基因型中，PS-1 mRNA与颞中皮层APP和APLP 2 mRNA及上级额叶皮层APP mRNA表达呈显著正相关，这可能反映了这些基因表达的共同调节，或反映了它们在相似的神经元群体中表达的事实。
The presenilin-1 (PS-1) gene on chromosome 14 carries mutations which cosegregate with early-onset familial Alzheimer's disease, We quantified PS-1 mRNA in post-mortem mid-temporal and superior frontal cortices from 14 Alzheimer's disease subjects, 9 non-demented controls and 5 subjects with other neurological diseases using solution hybridisation-RNase protection assay, APP and APLP2 mRNAs had previously been quantified in these samples (Johnston et al, (1996) Mel. Brain Res., in press) and subjects were apolipoprotein E (APOE) genotyped, There mere no significant differences between PS-1 mRNA levels per pg total RNA in mid-temporal or superior frontal cortices of the Alzheimer's disease subjects, compared to controls, PS-1 mRNA levels corresponded to 10% of total APP and 30% of APLP2 mRNA levels, and were not significantly affected by age, post-mortem delay, tissue pH, or APOE genotype, PS-1 mRNA showed significant positive correlations with APP and APLP2 mRNA levels in mid-temporal cortex and with APP mRNA in superior frontal cortex, This may reflect a co-regulation of the expression of these genes, or the fact that they are expressed in similar neuronal populations.