Georgian white coat color of red fox (Vulpes vulpes) maps to fox chromosome 2 in the region containing KIT gene.
Georgian white coat color of red fox (Vulpes vulpes) maps to fox chromosome 2 in the region containing KIT gene.
复制标题
红狐（Vulpes vulpes）的格鲁吉亚白毛色映射到狐狸2号染色体上含有KIT基因的区域。
DOI：
10.1111/age.12439
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发表时间：
2016
期刊：
影响因子：
2.4
通讯作者：
Trut,LyudmilaN
中科院分区：
文献类型：
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作者：
Kukekova,AnnaV;Johnson,JenniferL;Kharlamova,AnastasiyaV;Vladimirova,AnastasiyaV;Shepeleva,DaryaV;Gulevich,RimmaG;Trut,LyudmilaN
赤狐的养殖始于世纪后期的加拿大东部。[1]通过狐狸养殖场的繁殖，已经鉴定出了几种新的毛色变体，其中格鲁吉亚白色（GW）变体于1943年在格鲁吉亚的巴库里亚尼农场首次被描述。2、3 GW狐是通过GW和标准银狐交配产生的，并且对于GW等位基因是杂合的。它们的特征是被毛为白色，眼睛为棕色，鼻子为黑色，头部、沿着脊柱和身体其他部位有黑色斑点（图1）。在两只GW狐狸之间的繁殖中，GW等位基因纯合的后代代表性不足，这是由于一些纯合胚胎在着床前死亡。3. GW等位基因纯合的活产后代几乎全是白色，并在1-2月龄时死亡。3有趣的是，GW狐狸之间的GW纯合子后代的短缺可以通过在怀孕期间对母体进行额外的照明来减少。3血液样本采集自俄罗斯新西伯利亚俄罗斯科学院细胞学和遗传学研究所通过GW和标准银繁殖产生的13个三代家系（88只个体，其中第三代55只）。使用DNA Maxi Blood Kit（Qiagen）提取DNA。如所述，用224个犬微卫星（表S1）对家系进行基因分型。4-6检查所有基因型的孟德尔分离并手动校正错误。使用CRI-MAP版本2.5037，8的两点函数的连锁作图鉴定了GW与狐狸2号染色体（VVU 2）上的标记物之间的最显著连锁：FH 2890（θ= 0.0; LOD= 16.26）、FH 3006（θ= 0.0; LOD= 13.85）和AHT 121（θ= 0.0; LOD= 11.44）（表S2）。多点连锁作图和单倍型分析将GW位点置于区间内
The farm breeding of red foxes began in Eastern Canada in the late 19th century. 1 Through fox farm breeding, several new coat color variants have been identified, with the Georgian white (GW) morph first being described at Bakuriany farm in Georgia in 1943. 2, 3 GW foxes are produced by breeding GW and standard silver foxes and are heterozygous for the GW allele. They are characterized by white coat color, brown eyes, a black nose and black spots on the head, along the spine and on some other parts of the body (Fig. 1). In a breeding between two GW foxes, offspring homozygous for the GW allele are underrepresented, which was attributed to the death of some homozygous embryos before implantation. 3 The offspring homozygous for the GW allele that are born alive are almost completely white and die at 1–2 months of age. 3 Interestingly, the shortage of GW homozygous offspring in breedings between GW foxes can be reduced by additional illumination of the dam during pregnancy. 3Blood samples were collected from 13 three-generation pedigrees produced by breeding GW and standard silver foxes (88 individuals, including 55 foxes in the third generation) at the Institute of Cytology and Genetics of the Russian Academy of Sciences in Novosibirsk, Russia. DNA was extracted using DNA Maxi Blood Kit (Qiagen). The pedigrees were genotyped with 224 dog microsatellites (Table S1) as described. 4–6 All genotypes were checked for Mendelian segregation and manually corrected for errors. Linkage mapping using the Twopoint function of CRI-MAP version 2.5037, 8 identified the most significant linkage between GW and markers on fox chromosome 2 (VVU2): FH2890 (θ= 0.0; LOD= 16.26), FH3006 (θ= 0.0; LOD= 13.85) and AHT121 (θ= 0.0; LOD= 11.44)(Table S2). Multipoint linkage mapping and haplotype analysis placed the GW locus in the interval