Calcium-induced fusion and lateral phase separations in phosphatidylcholine-phosphatidylserine vesicles. Correlation by calorimetric and fusion measurements
Calcium-induced fusion and lateral phase separations in phosphatidylcholine-phosphatidylserine vesicles. Correlation by calorimetric and fusion measurements
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磷脂酰胆碱-磷脂酰丝氨酸囊泡中钙诱导的融合和横向相分离。
DOI：
10.1021/bi00309a019
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
J. Gagné
中科院分区：
文献类型：
--
作者：
J. Silvius;J. Gagné
材料和方法用于纯DMPC、DMPS、DEPC、DEPS、DOPC和DOPS的制备和纯化、用于脂质分散体的量热分析和用于囊泡融合测定的程序已在先前的论文（Silvius & Gagné，1984）中描述。通过Wilschut等人的程序制备用于融合测定的大单层囊泡（LUV）。（1980年）。通过Wilschut等人的程序测定囊泡融合。（1980）或Struck et al.（1981），由Hoekstra（1982 a）修改，如前一篇论文（Silvius & Gagné，1984）所述。用于量热法的脂质分散体通过从苯中冷冻干脂质组分（并且，对于向其中加入钙的样品，钙离子载体A23187以1/250脂质的摩尔比）来制备。通过涡旋将冻干的混合物分散在200 mM NaCl、5 mM Tes、5 mM组氨酸和1.0 mM EDTA（pH 7.4）（“无钙缓冲液”）中，或分散在100 mM NaCl、5 mM Tes和5 mM组氨酸（pH 7.4）中，向其中加入氯化钙（从1 mM Tes到1.0 mM EDTA）。
Materials and MethodsThe procedures used for the preparation and purification of pure DMPC, DMPS, DEPC, DEPS, DOPC, and DOPS, for the calorimetric analysis of lipid dispersions, and for the assays of vesicle fusion have been described in the preceding paper (Silvius & Gagné, 1984). Large unilamellar vesicles (LUV) were prepared for fusion assays bythe procedure of Wilschut et al.(1980). Vesicle fusion was assayed by the procedure of Wilschut et al.(1980) or the procedure of Struck et al.(1981) as modified by Hoekstra (1982a), as described in the precedingpaper (Silvius & Gagné, 1984). Lipid dispersions for calorimetry were prepared by colyophilization of the lipid components (and, for samples to which calcium was added, the calcium ionophore A23187 at a molar ratio of 1 per 250 lipids) from benzene. The lyophilized mixtures were dispersed by vortexing in200 mM NaCl, 5 mM Tes, 5 mM histidine, and 1.0 mM EDTA, pH 7.4 (“calcium-free buffer”), or in 100 mM NaCl, 5 mM Tes, and 5 mM histidine, pH 7.4, to which calcium chloride (from a 1
影响因子：
2.9
作者：
STRUCK, DK;HOEKSTRA, D;PAGANO, RE
通讯作者：
PAGANO, RE