Enhancement of transcriptional activity of DNA complexes by amphoteric PEG derivative
Enhancement of transcriptional activity of DNA complexes by amphoteric PEG derivative
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DOI：
10.1021/bm0504633
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发表时间：
2006-04-01
影响因子：
6.2
通讯作者：
Ito, T
中科院分区：
文献类型：
--
作者：
Koyama, Y;Yamashita, M;Ito, T
合成了具有氨基和羧酸侧链的水溶性PEG衍生物（PEG-AC），并对其转录和转染增强活性进行了探索。PEG-AC可沉积在DNA/聚阳离子复合物表面，形成表面电位略负的三元复合物。PEG-AC-包被在质粒/PEI复合物上明显增强了它们的转录活性，并且观察到高31倍的UTP消耗。两性PEG-AC可使紧密结合的DNA/PEI复合物松动，促进转录因子的接近。PEG-AC还明显提高了转基因在CHO细胞上的表达水平。
A water-soluble PEG derivative having both amino and carboxylic acid side chains (PEG-AC) was synthesized and explored for its transcription- and transfection-enhancing activity. PEG-AC could be deposited onto the surface of DNA/polycation complexes to form a ternary complex with slightly negative surface potential. PEG-AC-coating on the plasmid/PEI complexes obviously enhanced their transcriptional activity, and 31-fold higher consumption of UTP was observed. Amphoteric PEG-AC would loosen the tightly compacted DNA/PEI complex and facilitate the approach of transcriptional factors. PEG-AC also evidently improved the transgene expression level on the cultured CHO cells.