The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities
The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities
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DOI：
10.1094/mpmi.2004.17.4.428
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发表时间：
2004-04-01
影响因子：
3.5
通讯作者：
Jacobsen, E
中科院分区：
文献类型：
--
作者：
Huang, SW;Vleeshouwers, VGAA;Jacobsen, E
马铃薯（Solanum tuberosum L.）的R3位点对晚疫病的致病因子疫霉（Phytophthora infestans）的无毒分离株具有完全的抗性。R3位于11号染色体的远端部分，并在马铃薯作图群体中分离，从中可以获得饱和的扩增片段长度多态性图谱。利用1748株植物的种群，我们在R3位点构建了一个高分辨率的遗传图谱。利用精细定位和精确疾病检测相结合的方法，我们发现R3位点由两个具有不同特异性的基因组成。这两个基因R3a和R3b相距0.4 cM，都是从S. demissum（最具特征的种族特异性R基因的“供体”种）渗入到P . infestans的。在一株稻瘟病菌中发现了R3a和R3b之间的天然重组体。讨论了R3基因座与番茄12基因座的同源性。
The R3 locus of potato (Solanum tuberosum L.) confers full resistance to avirulent isolates of Phytophthora infestans, the causal agent of late blight. R3 resides in the distal part of chromosome 11 and segregates in a potato mapping population, from which a well-saturated amplified fragment length polymorphism map is available. Using a population of 1,748 plants, we constructed a high-resolution genetic map at the R3 locus. Using the combination of fine mapping and accurate disease testing with specific P infestans isolates, we detected that the R3 locus is composed of two genes with distinct specificities. The two genes R3a and R3b are 0.4 cM apart and have both been introgressed from S. demissum, the 'donor' species of most characterized race-specific R genes to P infestans. A natural recombinant between R3a and R3b was discovered in one accession of S. demissum. The synteny between the R3 locus and the tomato 12 locus is discussed.