Dynamics of bacteriorhodopsin 2D crystal observed by high-speed atomic force microscopy
Dynamics of bacteriorhodopsin 2D crystal observed by high-speed atomic force microscopy
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DOI：
10.1016/j.jsb.2009.04.011
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发表时间：
2009-08-01
影响因子：
3
通讯作者：
Ando, Toshio
中科院分区：
文献类型：
--
作者：
Yamashita, Hayato;Voitchovsky, Kislon;Ando, Toshio
我们用高速原子力显微镜研究了紫膜中天然晶相自由界面细菌视紫红质(BR)分子的动力学。我们的结果揭示了由BR分子的缔合和解离引起的晶体边缘的时间波动，最主要的是预形成的三聚体。对解离动力学的分析得到了三聚体间单键能的估计值为-0.9kcal/mol。单个结合的三聚体的旋转运动表明，三聚体间的键涉及W10-W12色氨酸残基。(C)2009 Elsevier Inc.保留所有权利。
We have used high-speed atomic force microscopy to study the dynamics of bacteriorhodopsin (bR) molecules at the free interface of the crystalline phase that occurs naturally in purple membrane. Our results reveal temporal fluctuations at the crystal edges arising from the association and dissociation of bR molecules, most predominantly pre-formed trimers. Analysis of the dissociation kinetics yields an estimate of the inter-trimer single-bond energy of -0.9 kcal/mol. Rotational motion of individual bound trimers indicates that the inter-trimer bond involves W10-W12 tryptophan residues. (C) 2009 Elsevier Inc. All rights reserved.