Structure and expression of the Arabidopsis thaliana homeobox gene Athb-12
Structure and expression of the Arabidopsis thaliana homeobox gene Athb-12
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DOI：
10.1006/bbrc.2001.4904
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发表时间：
2001-06-01
影响因子：
3.1
通讯作者：
Chun, JY
中科院分区：
文献类型：
--
作者：
Lee, YH;Oh, HS;Chun, JY
我们分离了拟南芥同源盒基因AthB-12，确定了其结构和激活结构域，证明了其启动子在脱落酸(ABA)处理下是可诱导的，并对其转录产物的细胞分布进行了表征。该基因的单个内含子中断了亮氨酸拉链结构域。在烟草转基因植株中，AthB-12的5‘调控区可以驱动β-葡萄糖醛酸酶(GUS)的表达。用原位杂交法进一步检测ABA诱导过程中ATSB-12基因的表达，以确定其转录产物在细胞内的分布。观察到一种复杂的Athb-12表达模式，通常与发育中的血管组织的区域有关。对由AthB-12构建的嵌合体和酿酒酵母转录因子GAL4的DNA结合域的分析表明，AthB-12的活化域位于C-末端(180到235个氨基酸)。综上所述，我们的数据表明，AthB-12是一个重要的转录激活因子，在调控某些发育过程以及涉及ABA介导的基因表达的植物对水分胁迫的反应中发挥重要作用。(C)2001年学术出版社。
We have isolated the Arabidopsis thaliana homeobox gene Athb-12, determined its structure and activation domain, demonstrated that its promoter is inducible in response to abscisic acid (ABA) treatment, and characterized the cellular distribution of its transcripts. The single intron of the gene interrupted the leucine-zipper domain region. The 5 ' regulatory region of Athb-12 can drive beta -glucuronidase (GUS) expression in tobacco transgenic plants. Athb-12 gene expression was further examined using in situ hybridization to determine the cellular distribution of Athb-12 transcripts during ABA induction. A complex pattern of Athb-12 expression was observed, often associated with regions of developing vascular tissues. Analysis of chimeras constructed from Athb-12 and the DNA-binding domain of the Saccharomyces cerevisiae transcription factor GAL4 revealed that the activation domain of Athb-12 lies in the C-terminal region (amino acids 180 to 235). Taken together, our data suggest that Athb-12 is a transcriptional activator important in regulating certain developmental processes as well as in the plant's response to water stress involving ABA-mediated gene expression. (C) 2001 Academic Press.