Molecular characterization of infectious bursal disease virus (IBDV): Diversity of very virulent IBDV in Tanzania
Molecular characterization of infectious bursal disease virus (IBDV): Diversity of very virulent IBDV in Tanzania
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DOI：
10.1007/s00705-006-0898-5
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发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
Fukushi, H.
中科院分区：
文献类型：
--
作者：
Kasanga, C. J.;Yamaguchi, T.;Fukushi, H.
测定了 2001 年至 2004 年在坦桑尼亚分离的 14 种传染性法氏囊病病毒 (IBDV) 的 VP2 高变区 (VP2-HVR) 的核苷酸序列。系统发育分析表明，分离株分化为两种基因型，属于强毒力（W）型。在系统发育树中，一种基因型的菌株聚集在一个不同的群体中，与西非分离的一些菌株密切相关，核苷酸相似性为96.1-96.8%，而另一种基因型的菌株聚集在欧洲/亚洲VV型中，核苷酸相似性范围为97.5-99.3%。两种基因型广泛分布于整个坦桑尼亚，并且在位置222(A)、242(1)、256(1)、294(1)和299(S)处具有保守的推定毒力标记氨基酸(aa)。我们的研究结果首次证明坦桑尼亚存在非洲和欧洲/亚洲 VV-IBDV 变种。
Nucleotide sequences of the VP2 hypervariable region (VP2-HVR) of 14 infectious bursal disease viruses (IBDVs) isolated in Tanzania from 2001 to 2004 were determined. Phylogenetic analysis showed that the isolates diverged into two genotypes and belonged to the very virulent (W) type. In the phylogenetic tree, strains in one genotype clustered in a distinct group and were closely related to some strains isolated in western Africa, with nucleotide similarities of 96.1-96.8%, while strains in another genotype were clustered within the European/Asian VV type with nucleotide similarities ranging from 97.5 to 99.3%. Both genotypes were widely distributed throughout Tanzania, and had conserved putative virulence marker amino acids (aa) at positions 222(A), 242(l), 256(l), 294(l) and 299(S). Our findings demonstrate for the first time the existence of both African and European/Asian VV-IBDV variants in Tanzania.