Studies of in vivo distribution of bovine herpesvirus type 4 in the natural host
Studies of in vivo distribution of bovine herpesvirus type 4 in the natural host
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DOI：
10.1128/jcm.34.5.1091-1095.1996
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发表时间：
1996-05-01
影响因子：
9.4
通讯作者：
Belak, S
中科院分区：
文献类型：
--
作者：
Egyed, L;BallagiPordany, A;Belak, S
通过检测实验感染小牛的鼻和结膜渗出物、外周血白细胞和各种器官，检查了牛疱疹病毒4型（BHV-4）的体内分布。对于病毒检测，应用巢式PCR测定、病毒分离和免疫组织化学。神经系统和肌肉中没有病毒DNA。肝脏和肠淋巴结含有少量的病毒（每1 μ g细胞DNA少于2个拷贝）。肠、扁桃体、胸腺和肾组织中含有更多的病毒DNA拷贝（每1 μ g细胞DNA中有5至50个拷贝）。在脾、肺、气管和鼻上皮中发现了最高量的BHV-4 DNA（每1 μ g细胞DNA 50至500个拷贝）。通过接种后（p.i.）第48天证明病毒在接种小牛后立即开始在这些细胞中复制，并且在感染的7至8周期间发生了密集的病毒生长。感染病毒的淋巴细胞数在p.i.第22 - 26天，此后缓慢下降。病毒感染的细胞仅在p.i.第48天免疫组化。蛋白质印迹（免疫印迹）检测到针对29种BHV-4蛋白中9种的免疫反应迹象。
The in vivo distribution of bovine herpesvirus type 4 (BHV-4) was examined by testing nasal and conjunctival exudates, peripheral blood leukocytes, and various organs of experimentally infected calves. For virus detection, a nested PCR assay, virus isolation, and immunohistochemistry were applied. The nervous system and the muscles were free of viral DNA. Liver and intestinal lymph nodes contained low amounts of virus (less than two copies per 1 mu g of cellular DNA). Intestinal, tonsil, thymus, and kidney tissues contained more viral DNA copies (5 to 50 copies per 1 mu g of cellular DNA). The highest amounts of BHV-4 DNA (50 to 500 copies per 1 mu g of cellular DNA) were found in the spleen, lungs, trachea, and nasal epithelium. Amplification of DNA from blood lymphocytes through postinoculation (p.i.) day 48 proved that the virus started to replicate in these cells immediately after inoculation of the calves and that intensive virus growth took place during the 7 to 8 weeks of the infection. The number of virus-infected lymphocytes reached the maximum on p.i. days 22 to 26 and slowly declined thereafter. Virus-infected cells were found only in the spleen on p.i. day 48 by immunohistochemistry. Western blotting (immunoblotting) detected signs of an immune response against 9 of the 29 BHV-4 proteins.