Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells.
Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells.
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悬浮在胶原蛋白凝胶中的上皮可以失去极性，并表达迁移间充质细胞的特征。
DOI：
10.1083/jcb.95.1.333
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发表时间：
1982-10
影响因子：
7.8
通讯作者：
Hay, E D
中科院分区：
文献类型：
--
作者：
Greenburg, G;Hay, E D
上皮间充质转化和上皮细胞极性在体外的这项研究表明，环境条件可以有一个深刻的影响上皮表型，细胞形状，和极性所表示的顶面和底面的存在。许多不同的成人和胚胎上皮细胞悬浮在天然胶原凝胶内。在这些条件下，细胞伸长，从外植体分离，并作为个体细胞在三维网格内迁移，这是分化良好的上皮细胞的一种先前未知的特性。对成人和胚胎前透镜上皮细胞进行了详细的研究。来自顶端表面的伸长细胞发育出具有迁移细胞特征的伪足和丝状伪足，并获得与体内间充质细胞几乎无法区分的形态和超微结构。从这些实验可以得出结论，三维胶原凝胶可以促进分化的上皮细胞的分泌细胞器的解离、迁移和获得，并且可以消除原始上皮的顶-基底细胞极性特征。
This study of epithelial-mesenchymal transformation and epithelial cell polarity in vitro reveals that environmental conditions can have a profound effect on the epithelial phenotype, cell shape, and polarity as expressed by the presence of apical and basal surfaces. A number of different adult and embryonic epithelia were suspended within native collagen gels. Under these conditions, cells elongate, detach from the explants, and migrate as individual cells within the three-dimensional lattice, a previously unknown property of well-differentiated epithelia. Epithelial cells from adult and embryonic anterior lens were studied in detail. Elongated cells derived from the apical surface develop pseudopodia and filopodia characteristic of migratory cells and acquire a morphology and ultrastructure virtually indistinguishable from that of mesenchymal cells in vivo. It is concluded from these experiments that the three-dimensional collagen gel can promote dissociation, migration, and acquisition of secretory organelles by differentiated epithelial cells, and can abolish the apical-basal cell polarity characteristic of the original epithelium.