HPLC qualitative amino acid analysis in the clinical laboratories.
HPLC qualitative amino acid analysis in the clinical laboratories.
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临床实验室中的 HPLC 定性氨基酸分析。
DOI：
10.1093/ajcp/87.5.614
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发表时间：
1987
影响因子：
3.5
通讯作者：
J. Rudy
中科院分区：
文献类型：
--
作者：
J. Rutledge;J. Rudy
作者开发了一种用于临床实验室的快速高压液相色谱法氨基酸定性分析系统。稳定的丹磺酰氯衍生物的反相、线性梯度、高压液相色谱法是用C-18柱在30分钟内完成的，用紫外检测。将所得色谱图与反映血清和尿液氨基酸高正常水平的模板色谱图进行比较。与先天性代谢缺陷相关的氨基酸的大幅升高很容易确定，通过相对保留时间鉴定特定氨基酸。该技术为氨基酸病的研究提供了一种快速和特异的定性方法，并使用通用和标准的液相色谱设备。
The authors have developed a rapid system for qualitative amino acid analysis by high-pressure liquid chromatography for use in the clinical laboratory. Reverse-phase, linear gradient, high-pressure liquid chromatography of stable dansyl chloride derivatives is accomplished with a C-18 column over 30 minutes, with ultraviolet detection. The resulting chromatograms are compared with template chromatograms that reflect high normal levels of serum and urine amino acids. Large elevations of amino acids associated with inborn errors of metabolism are easily pinpointed, with identification of specific amino acids made by the relative retention times. This technic makes available a rapid and specific qualitative method for investigation of aminoacidopathies and uses versatile and standard liquid chromatography equipment.