Comparison of captive and pulsating bubble surfactometers with use of lung surfactants.
Comparison of captive and pulsating bubble surfactometers with use of lung surfactants.
复制标题
使用肺表面活性剂的捕获和脉动气泡表面活性剂的比较。
DOI：
10.1152/jappl.1994.76.4.1425
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Clements,JA
中科院分区：
文献类型：
--
作者：
Putz,G;Goerke,J;Taeusch,HW;Clements,JA
用脉动气泡表面测定仪(PBS)和俘获气泡表面测定仪(CBS)对不同表面活性的表面活性剂的表面张力进行了比较。对兔肺灌洗表面活性物质(60000×平均g，60min)、牛表面活性物质提取物(Survanta)和合成脂质表面活性物质混合物(二棕榈酰磷脂酰胆碱-卵磷脂酰甘油-棕榈酸)进行了研究，其浓度为1.25 mg磷脂/毫升。使用PBS时，要么不加任何修改，要么保持样品室毛细管干燥，样品以最大气泡尺寸吸附(5min)。CBS的使用方式模仿了未经修改的PBS。我们发现，这三种技术在60K的第一个循环中都显示出较低的表面张力。对于Survanta，CBS和改性PBS在第一个循环中报告了低表面张力，而未改性PBS在10个循环内没有达到这一目标。对于合成的脂类表面活性剂混合物，只有CBS在10个循环内测量到低表面张力。视频观察表明，改性的PBS比未改性的PBS性能更好，因为保持毛细管干燥可以防止表面膜在循环过程中占据这一大表面，从而允许更大的面积压缩。
We compared surface tension measures of surfactants with various surface activities by using a pulsating bubble surfactometer (PBS) and a captive bubble surfactometer (CBS). Rabbit lung lavage surfactant (60,000 x average g for 60 min), bovine surfactant extract (Survanta), and a synthetic lipid surfactant mixture (dipalmitoylphosphatidylcholine-egg phosphatidylglycerol-palmitic acid) were studied at 1.25 mg phospholipid/ml. The PBS was used either unmodified according to manufacturer's instructions or with the sample chamber capillary kept dry and the sample adsorbing at maximum bubble size (5 min). The CBS was used in a manner that imitated the unmodified PBS. We found that all three techniques indicated low surface tension on the first cycle for 60K. For Survanta, the CBS and the modified PBS reported low surface tension on the first cycle, whereas the unmodified PBS did not achieve this within 10 cycles. For the synthetic lipid surfactant mixture, only the CBS measured low surface tension within 10 cycles. Video observations indicate that the modified PBS performs better than the unmodified PBS because keeping the capillary dry prevents surface film from occupying this large surface during cycling, thereby allowing larger area compressions.