OSTEONECTIN CDNA SEQUENCE REVEALS POTENTIAL BINDING REGIONS FOR CALCIUM AND HYDROXYAPATITE AND SHOWS HOMOLOGIES WITH BOTH A BASEMENT-MEMBRANE PROTEIN (SPARC) AND A SERINE PROTEINASE-INHIBITOR (OVOMUCOID)
OSTEONECTIN CDNA SEQUENCE REVEALS POTENTIAL BINDING REGIONS FOR CALCIUM AND HYDROXYAPATITE AND SHOWS HOMOLOGIES WITH BOTH A BASEMENT-MEMBRANE PROTEIN (SPARC) AND A SERINE PROTEINASE-INHIBITOR (OVOMUCOID)
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DOI：
10.1073/pnas.85.9.2919
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发表时间：
1988-05-01
影响因子：
11.1
通讯作者：
TERMINE, JD
中科院分区：
文献类型：
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作者：
BOLANDER, ME;YOUNG, MF;TERMINE, JD
骨粘连蛋白是发育中骨骼的一种重要非胶原蛋白。对从牛骨细胞Gt11表达文库中分离的编码骨粘连蛋白的2150个碱基对的cDNA进行测序，并通过与蛋白质序列数据进行比较来鉴定。核苷酸序列预测骨连接素含有304个氨基酸，包括17个残基的信号肽。推导的蛋白质序列的分析表明，分泌的蛋白质含有至少四个不同的结构域。在蛋白质的氨基末端的酸性区域似乎是一个潜在的羟基磷灰石结合位点。其次是第二个结构域，富含半胱氨酸，与火鸡卵类粘蛋白和其他丝氨酸蛋白酶抑制剂中富含半胱氨酸的结构域具有序列同源性。两个序列与“EF手”的中央钙结合环同源，因此具有成为高亲和力钙结合位点的潜力，位于蛋白质羧基末端一半内的另外两个结构域中。 最后，骨粘连蛋白序列显示出与另一种蛋白质接近的同一性（> 90%），所述蛋白质是由小鼠壁内胚层分泌的分泌蛋白，酸性且富含半胱氨酸。这些数据表明，骨粘连蛋白，一种存在于骨和其他选定组织中的蛋白质，是一种多功能蛋白质。
Osteonectin is a prominent noncollagenous protein of developing bone. A 2150-base-pair cDNA coding for osteonectin, isolated from a bovine bone cell .lambda.gt11 expression library, was sequenced and identified by comparison with protein sequence data. The nucleotide sequence predicts that osteonectin contains 304 amino acids, including a 17-residue signal peptide. Analysis of the deduced protein sequence suggests that the secreted protein contains at least four distinct structural domains. An acidic region at the amino terminus of the protein appears to be a potential hydroxyapatite-binding site. This is followed by a second domain, rich in cysteine, that shows sequence homology with cysteine-rich domains in turkey ovomucoid and other serine proteinase inhibitors. Two sequences homologous with central calcium-binding loops of "EF hands" and thus having potential to be high-affinity calcium-binding sites are located in two other domains within the carboxyl-terminal half of the protein. Finally, the osteonectin sequence shows near identity (> 90%) with another protein, SPARC (secreted protein, acidic and rich in cysteine), secreted by mouse parietal endoderm. These data suggest that osteonectin, a protein present in bone and other selected tissues, is a multifunctional protein.