Cloning, chromosome mapping and expression characteristics of porcine ANGPTL3 and -4
Cloning, chromosome mapping and expression characteristics of porcine ANGPTL3 and -4
复制标题
DOI：
10.1159/000091927
复制
发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Yang, Z. Q.
中科院分区：
文献类型：
--
作者：
Feng, S. Q.;Chen, X. D.;Yang, Z. Q.
血管生成素样蛋白 3 和 -4（ANGPTL3 和 -4）是血管生成素样蛋白 (ANGPTL) 的两个成员，它们具有血管生成素家族的特征结构，但不能与 TIE2 受体结合。据报道，它们都通过抑制脂蛋白脂肪酶（LPL）的活性来影响脂质代谢。在此，我们报道了猪ANGPTL3和-4的cDNA克隆、染色体定位和表达分析。序列分析显示，ANGPTL3含有1,389 bp的开放阅读框，编码462个氨基酸，ANGPTL4含有1,239 bp的编码区，编码412个氨基酸。猪ANGPTL3推导的氨基酸序列与人和小鼠分别具有83%和73.7%的同一性，ANGPTL4与人和小鼠分别具有79.4%和77.7%的氨基酸同一性。通过辐射混合作图，将猪 ANGPTL3 和 -4 分别映射到 6q31 -> q35 和 2q21 -> q24 区域。组织分布分析表明，猪ANGPTL3 mRNA仅在肝脏中表达，猪ANGPTL4在白色脂肪组织中广泛表达，丰度最高。此外，遗传性肥胖猪肝脏中ANGPTL3和-4的mRNA水平以及白色脂肪组织中ANGPTL4的mRNA水平显着高于瘦猪。这是首次在猪体内报道ANGPTL3和-4的分子克隆和表征，这将有助于更好地了解ANGPTLs在脂质代谢中的作用。版权所有 (c) 2006 S. Karger AG，巴塞尔。
Angiopoietin-like protein 3 and -4 (ANGPTL3 and -4) are two members of angiopoietin-like proteins (ANGPTLs), which have the signature structure of the angiopoietin family but cannot bind to the TIE2 receptor. It has been reported that they both affect lipid metabolism by inhibiting the activity of lipoprotein lipase (LPL). Here we report the cDNA cloning, chromosome mapping and expression analysis of ANGPTL3 and -4 in pigs. Sequence analysis shows that ANGPTL3 contains an open reading frame of 1,389 bp, which encodes 462 amino acids, and ANGPTL4 contains a coding region of 1,239 bp, which encodes 412 amino acids. Porcine ANGPTL3 deduced amino acid sequence shares 83% and 73.7% identity with human and mouse, respectively, and ANGPTL4 shares 79.4% and 77.7% amino acid identity with human and mouse, respectively. Porcine ANGPTL3 and -4 were mapped to the 6q31 -> q35 and 2q21 -> q24 region, respectively, by radiation hybrid mapping. Tissue distribution analysis indicated that porcine ANGPTL3 mRNA was exclusively expressed in liver, and porcine ANGPTL4 was ubiquitously expressed with the highest abundance in white adipose tissue. Furthermore, the mRNA level of ANGPTL3 and -4 in liver and the mRNA level of ANGPTL4 in white adipose tissue were significantly higher in genetically obese pigs than in their lean counterparts. This is the first report of molecular cloning and characterization of ANGPTL3 and -4 in pigs, which will be helpful for a better understanding of the role of ANGPTLs in lipid metabolism. Copyright (c) 2006 S. Karger AG, Basel.