Protective effect of quercetin against oxidative stress induced by oxytetracycline in muscle of silver catfish
Protective effect of quercetin against oxidative stress induced by oxytetracycline in muscle of silver catfish
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DOI：
10.1016/j.aquaculture.2017.10.043
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发表时间：
2018-02
期刊：
影响因子：
4.5
通讯作者：
T. Pês;E. Saccol;Érika P. Londero;Caroline A. Bressan;Giovana M. Ourique;T. Rizzetti;O. Prestes;R. Zan
中科院分区：
文献类型：
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作者：
T. Pês;E. Saccol;Érika P. Londero;Caroline A. Bressan;Giovana M. Ourique;T. Rizzetti;O. Prestes;R. Zan
研究了槲皮素对土霉素（OTC）诱导的氧化应激和银鲶（Rhamdia quelen）肌肉中土霉素残留的保护或改善作用。将鱼分成四个不同的实验组，并接受以下实验饮食14或21天：（i）-对照;（ii）-槲皮素;（iii）-OTC;或（iv）-槲皮素加OTC。在实验结束时收集肌肉样品，并分析氧化剂/抗氧化剂状态和OTC残留物的存在。鱼肉中土霉素含量低于定量限。在14日龄和21日龄时，OTC处理组的肌肉中脂质过氧化和蛋白质羰基含量显著增加。槲皮素治疗避免了接受槲皮素加OTC饮食的组的这种增加。超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性下降，在OTC处理的鱼在14天。槲皮素能有效地防止添加OTC饲料组GPx活性的下降。处理21天后，OTC处理的鱼的肌肉中的SOD，GPx和谷胱甘肽S-转移酶的活性降低。槲皮素能阻止OTC+槲皮素组SOD活性的下降。槲皮素组谷胱甘肽还原酶活性在治疗14和21天时较高。槲皮素组和槲皮素+OTC组的抗坏血酸（AA）含量在治疗14天后增加。处理21天后，在OTC处理的鱼的肌肉中的AA和非蛋白硫醇含量下降。因此，本研究表明，槲皮素保护肌肉免受OTC诱导的氧化应激，这表明它可能是一种有用的化合物，以尽量减少需要OTC治疗的鱼类的不良反应。
Protective or ameliorative effects of quercetin on the oxytetracycline (OTC) induced oxidative stress and presence of OTC residues in the muscle of silver catfish (Rhamdia quelen) were assessed. Fish were divided into four different experimental groups and received the following experimental diets for 14 or 21 days: (i)-control; (ii)-quercetin; (iii)-OTC; or (iv)-quercetin plus OTC. Muscle samples were collected at the end of the experiment and analyzed for the oxidant/antioxidant status and presence of OTC residues. The OTC values in muscle of fish were lower than the quantification limit. There was a significant increase on lipid peroxidation and protein carbonyl content in the muscle of OTC-treated fish at 14 and 21 days. Treatment with quercetin avoided this increase in the groups that received diet with quercetin plus OTC. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities decreased in the muscle of OTC-treated fish at 14 days. Quercetin was able to avoid the decrease of GPx activity in the group that fed diet with quercetin plus OTC. After 21 days of treatment there was a decrease of SOD, GPx and glutathione S-transferase activities in the muscle of OTC-treated fish. Quercetin was able prevent the decrease of SOD activity in the group that received diet with quercetin plus OTC. The glutathione reductase activity was higher in quercetin group at 14 and 21 days of treatment. Ascorbic acid (AA) content increased in quercetin and quercetin plus OTC groups after 14 days of treatment. After 21 days of treatment, the AA and non-protein thiols content decreased in the muscle of OTC-treated fish. Thus, the present study demonstrates that quercetin protects the muscle from the OTC-induced oxidative stress, suggesting that it may be a useful compound to minimize the adverse effects in fish requiring treatment with OTC.