Nanosheet coating improves stability of human pluripotent stem cell culture on glass substrates
Nanosheet coating improves stability of human pluripotent stem cell culture on glass substrates
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DOI：
10.1016/j.bbrc.2023.01.077
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发表时间：
2023-02-09
影响因子：
3.1
通讯作者：
Fukuda，Atsushi
中科院分区：
文献类型：
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作者：
Sakata，Yuka;Zhang，Hong;Fukuda，Atsushi
使用各种类型的细胞进行高分辨率成像分析是解剖细胞功能的重要工具。一般来说，获得此类图像需要将细胞培养在玻璃基板上；然而，它常常导致细胞状态不稳定。在这项研究中，我们报告使用疏水性聚苯乙烯组成的纳米片和基质胶涂覆玻璃基板，可显着提高人类多能干细胞（hPSC）的活力。此外，纳米片涂层不会影响 hPSC 的转录组状态，使研究人员能够进行高分辨率成像测定。这些结果表明纳米片涂层有利于易受玻璃基质培养的细胞。使用纳米片涂层，我们发现，对于雌性细胞中 X 染色体失活 (XCI) 至关重要的 lnc RNAXIST 在每个 hPSC 系的细胞核中的扩散状态存在显着差异。总而言之，我们的研究提供了一种利用高分辨率成像技术研究生物学问题的新方法。
High-resolution imaging analysis using various types of cells is an essential tool for dissecting cell functions. Generally, obtaining such images requires the cells to be cultured on glass substrates; however, it often results in the unstable status of cells. In this study, we report that coating the glass substrate using nanosheet composed of hydrophobic polystyrene, with Matrigel, significantly improves the viability of human pluripotent stem cells (hPSCs). Moreover, the nanosheet coating does not affect the transcriptome status of hPSC and enables researchers to perform the high-resolution imaging assay. These results indicate that the nanosheet coating is beneficial to the cells vulnerable to glass substrate culture. Using the nanosheet coating, we revealed that the spreading status of lnc RNAXIST, essential for X-chromosome inactivation (XCI) in female cells, in the nuclei significantly differs in each hPSC line. Taken together, our study provides a novel method to investigate biological questions using high-resolution imaging techniques.