Metabolic products of microorganisms
Metabolic products of microorganisms
复制标题
DOI：
10.1007/bf00409681
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
W. Pache;H. Zähner
中科院分区：
文献类型：
--
作者：
W. Pache;H. Zähner
浓度为0.0 5μg/ml的博莱霉素对枯草芽孢杆菌全细胞蛋白质、核糖核酸和脱氧核糖核酸的合成有抑制作用。它正被表面活性化合物拮抗，并与脂蛋白结合。博来霉素在细胞内的结合尤其发生在细胞质膜上，对枯草芽孢杆菌的抑制作用被高浓度的钾盐(如0.2MKCl)逆转。这种逆转是钾盐所特有的。加入博莱霉素后，可观察到钾离子从细胞中排出。细胞质膜上K+-Na+激活的ATP酶不受博莱霉素的影响。在四氯化碳人工膜上，与钠和锂离子相比，博莱霉素对钾离子的选择性较低。由于硼酸从分子中分离出来，博莱霉素通过碱解和酸解降解而失去抗菌活性。
Boromycin, at a concentration of 0.05 μg/ml inhibits the synthesis of protein, RNA and DNA in whole cells of Bacillus subtilis. It is being antagonised by surface active compounds and is being bound to lipoprotein. Binding of the boromycin within the cell especially takes place at the cytoplasmic membrane.The inhibitory effect to Bacillus subtilis is being reversed by high concentration of potassium salts (e.g. 0.2 m KCl). The reversion is specific of potassium salts. After the adding of boromycin a discharge of potassium ions from the cells can be observed. The K+-Na+-activated ATP-ase of the cytoplasmic membrane is not influenced by boromycin. On an artificial membrane of carbon tetrachloride boromycin shows a low selectivity for potassium ions compared with sodium and lithium ions.The degradation of boromycin through alkaline and acid hydrolysis leads to a loss of antibiotic activity, due to the splitting off the boric acid from the molecule.