Organization of membrane-associated proteins in lipid bilayers
Organization of membrane-associated proteins in lipid bilayers
复制标题
脂质双层中膜相关蛋白的组织
DOI：
10.1140/epje/i2007-10272-6
复制
发表时间：
2008-02-01
影响因子：
1.8
通讯作者：
Ma, Y. -Q
中科院分区：
文献类型：
--
作者：
Liang, Q.;Ma, Y. -Q
生物膜中蛋白质的横向组织对于信号转导、内吞作用和膜运输等膜功能至关重要。当前生物膜科学的主要目标之一是揭示生物膜中膜相关蛋白组织的微观机制。在这里，我们通过结合自洽场理论和密度泛函理论来研究脂质双层中膜相关蛋白的结构组织。本研究可以同时考虑脂质的熵效应、由于脂质头基的存在而导致的膜相关蛋白的消耗效应以及界面相互作用的影响。通过改变脂质的体积分数，我们检查了对蛋白质组织的各种影响，并揭示了由于界面能和弱耗尽效应，在低脂质体积分数下出现了密堆积晶体结构，而在高脂质体积分数下则主要由于强耗尽效应而出现了带有支链的链结构。目前的结果可能为膜蛋白组织的进一步实验提供一些理论见解。
Lateral organization of proteins in biomembranes is vitally important to membrane functions such as signal transduction, endocytosis, and membrane trafficking. One of the major goals in current biomembrane science is to reveal the microscopic mechanism of membrane-associated protein organization in biomembranes. Here, we investigate the structural organization of membrane-associated proteins in lipid bilayers by combining self-consistent field theory with density functional theory. The present study can simultaneously take into account the entropy effect of lipids, depletion effect of membrane-associated proteins due to the presence of lipid headgroups as well as the effect of interfacial interaction. By varying the volume fraction of lipids, we examine various effects on protein organization, and reveal that a close-packed crystal structure appears at low lipid volume fractions due to interfacial energy and weak depletion effect, whereas a chain structure with branches occurs at high lipid volume fractions mainly due to strong depletion. The present results may provide some theoretical insight into further experiments on organization of membrane proteins.