Characterization and online detection of aromatic alkaloids in the ascidian Lissoclinum cf. badium by liquid chromatography/UV detection mass spectrometry
Characterization and online detection of aromatic alkaloids in the ascidian Lissoclinum cf. badium by liquid chromatography/UV detection mass spectrometry
复制标题
DOI：
10.1002/rcm.2833
复制
发表时间：
2007-01-01
影响因子：
2
通讯作者：
Yao, Xinsheng
中科院分区：
文献类型：
--
作者：
Liu, Hongwei;Gao, Hao;Yao, Xinsheng
建立了基于高效液相色谱-紫外二极管阵列检测和电喷雾电离离子阱串联质谱（HPLC-UVESI-MS/MS）的检测方法来研究从海鞘中提取的总生物碱。巴迪姆。具有多硫化物结构的芳香生物碱是从海鞘属 Lissoclinum、Eudistoma 和 Polycitor 中分离出来的主要生物活性成分。这些化合物具有多种重要的生物活性。研究了该类生物碱的ESI-MS裂解行为，以NH(CH3)(2)部分的消除为特征。使用反相 HPLC/UV-ESI-MS 可以在线分离和检测 25 种芳香生物碱。该方法提供的数据可用于检测海洋生物中具有生物活性的芳香生物碱。版权所有 (c) 2006 John Wiley & Sons, Ltd.
A detection method based on high-performance liquid chromatography coupled with ultraviolet diode-array detection and electrospray ionization ion trap tandem mass spectrometry (HPLC-UVESI-MS/MS) was developed to investigate the total alkaloids prepared from the ascidian Lissoclinum cf. badium. The aromatic alkaloids possessing polysulfide structures are the major bioactive constituents isolated from ascidians of the genera Lissoclinum, Eudistoma, and Polycitor. These compounds presented various important biological activities. The ESI-MS fragmentation behavior of this kind of alkaloids was studied, and the fragmentation was characterized by elimination of the NH(CH3)(2) moiety. The use of reversed-phase HPLC/UV-ESI-MS allowed the online separation and detection of 25 aromatic alkaloids. This approach provided data that can be used for detection of biologically active aromatic alkaloids from marine organisms. Copyright (c) 2006 John Wiley & Sons, Ltd.