Odour of limonene affects feeding behaviour in the blowfly, Phormia regina
Odour of limonene affects feeding behaviour in the blowfly, Phormia regina
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DOI：
10.1016/j.neulet.2008.09.021
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发表时间：
2008-11
影响因子：
2.5
通讯作者：
Atsushi Nakamura;Tomoyuki Suzuki;Daiki Taniguchi;Atsushi Matsuda;Manabu Tobeta;Tadashi Nakamura
中科院分区：
文献类型：
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作者：
Atsushi Nakamura;Tomoyuki Suzuki;Daiki Taniguchi;Atsushi Matsuda;Manabu Tobeta;Tadashi Nakamura
在进餐时的气味体验被认为会影响许多动物的食物偏好。为了研究气味对食欲的调节，我们建立了一个新的实验系统，该系统对以前的方法进行了改进，在该系统中，喂食含有d-柠檬烯的蔗糖的果蝇对普通蔗糖的食欲随后会降低。在这个新的实验系统中，苍蝇种群被分成两组：(1)“同时”组同时接触d-柠檬烯和蔗糖10min，(2)“单独”组单独接触蔗糖10min，1小时后，接触柠檬烯10min。实验并未影响“分开”组果蝇对蔗糖的食欲，但“同时”组果蝇对蔗糖的食欲明显降低，这种影响持续了≥3天。为了研究d-柠檬烯对食欲的调节是否基于长期记忆形成(蛋白质合成)，我们检测了蛋白质合成抑制剂放线菌酮的效果。在柠檬烯和蔗糖暴露1h后注射放线菌亚胺，可抑制“同时”组果蝇的食欲抑制。此外，为了排除d-柠檬烯通过味觉而不是气味发挥作用的可能性，我们研究了移除嗅觉器官、触角和上颌触须对d-柠檬烯调节食欲的影响。当嗅觉器官被移除时，“同时”组的果蝇没有观察到食欲下降，这表明它们有嗅觉。在我们新的有效的果蝇食欲调节系统中，显示了通过嗅觉检测d-柠檬烯来调节食欲。
Experiences of odours at meals are thought to affect food preference in many animals. To study appetite modulation by odours, we established a new experimental system based on the modification of a previous method, where flies fed sucrose flavoured with d-limonene subsequently showed reduced appetite to plain sucrose. In this new experimental system, a fly population was divided into two groups: (1) the “simultaneous” group of flies was exposed to d-limonene and sucrose simultaneously for 10min, and (2) the “separate” group of flies was exposed to sucrose alone for 10min, and 1h later, to limonene for 10min. The appetite of flies in the “separate” group for sucrose was unaffected by the experiment, but the appetite of flies in the “simultaneous” group was significantly decreased, and this effect lasted for ≥3 days. To investigate if this appetite modulation by d-limonene was based on long-term memory formation (protein synthesis), we examined the effects of the protein synthesis inhibitor, cycloheximide. Injection of cycloheximide 1h after exposure to limonene and sucrose inhibited the appetite suppression in the “simultaneous” group of flies. In addition, to exclude the possibility that d-limonene exerted its effect through taste, rather than odour, we examined the effect of removing of the olfactory organs, antennae and maxillary palps on appetite modulation by d-limonene. When the olfactory organs were removed, no reduction in appetite was observed in the flies in the “simultaneous” group, indicating olfaction. In our new and effective appetite-modulation system for flies, modulation of appetite by olfactory detection of d-limonene was shown.