A FLUORIMETRIC METHOD FOR THE ESTIMATION OF THE CRITICAL MICELLE CONCENTRATION OF SURFACTANTS
A FLUORIMETRIC METHOD FOR THE ESTIMATION OF THE CRITICAL MICELLE CONCENTRATION OF SURFACTANTS
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DOI：
10.1016/0003-2697(81)90006-3
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发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
BOCCHINI, V
中科院分区：
文献类型：
--
作者：
DEVENDITTIS, E;PALUMBO, G;BOCCHINI, V
本文研究了用可溶性荧光探针快速测定表面活性剂临界胶束浓度（cmc）的可能性。研究了非离子或不同电荷表面活性剂对阴离子8-苯胺-1-萘磺酸镁盐（ANS）和阳离子罗丹明6G荧光性能的影响。cmc评价的可能性取决于染料-洗涤剂对的适当选择。ANS必须与阴离子表面活性剂一起使用；另一方面，罗丹明6G必须与阳离子洗涤剂一起使用。ANS和罗丹明6G已被证明对两性离子或非离子表面活性剂都有效。ANS荧光随表面活性剂浓度的增加或罗丹明6G的减少给出两条直线，其交点表示洗涤剂的cmc。在所有这些条件下，荧光探针不干扰胶束过程。荧光探针在等吸点的激发不影响洗涤剂cmc的评价。该方法适用于线性或类固醇表面活性剂，并且在很宽的浓度范围内与cmc值无关。
The present work investigates the possibility of a rapid estimation of critical micelle concentration (cmc) of surfactants by means of soluble fluorescent probes. The effect of nonionic or differently charged surfactants on the fluorescent properties of the anionic 8-anilino-1-naphtalenesulfonic acid magnesium salt (ANS) or cationic rhodamine 6G has been investigated. The possibility of cmc evaluation depends on the appropriate selection of the dye-detergent couple. ANS has to be used with anionic surfactants; on the other hand, rhodamine 6G has to be used with cationic detergents. Both ANS and rhodamine 6G have been proved to be effective with either zwitterionic or nonionic surfactants. Plots of ANS fluorescence increase or rhodamine 6G decrease vs surfactant concentration give two straight lines whose intersection indicates the cmc of the detergent. Under all these conditions the fluorescent probe does not interfere with the micellization process. Excitation of the fluorescent probes at the isosbestic point does not affect the evaluation of the cmc of the detergent. The method applies for linear or steroid surfactants and is independent of the cmc value within a wide range of concentrations.