Genomics of the 35-kb pvd locus and analysis of novel pvdIJK genes implicated in pyoverdine biosynthesis in Pseudomonas aeruginosa
Genomics of the 35-kb pvd locus and analysis of novel pvdIJK genes implicated in pyoverdine biosynthesis in Pseudomonas aeruginosa
复制标题
DOI：
10.1111/j.1574-6968.2000.tb09276.x
复制
发表时间：
2000-09-01
影响因子：
2.1
通讯作者：
Levesque, RC
中科院分区：
文献类型：
--
作者：
Lehoux, DE;Sanschagrin, F;Levesque, RC
在铜绿假单胞菌菌株 PAO1 的 6.2-Mb 染色体中，在 pvdD 上游发现了新的推定的吡弗丁合成酶 pvdIJK 基因。这些基因形成了与吡维丁生物合成有关的基因座。序列分析显示，这些基因的产物与PvdD有43%、60%和57%的同一性。 PvdIJK 被认为与 pyoverdine（一种铁载体螯合 Fe3+）的合成有关。通过基因破坏诱变获得pvdI突变体并通过Southern杂交证实。产生的 pvdI 突变体在补充有铁螯合剂 2,2-联吡啶的固体培养基上没有显着生长；而PvdI(-)表型消除了pyoverdine荧光。通过测量慢性肺部感染大鼠模型中铜绿假单胞菌野生型和突变株的体内生长，以及测量中性粒细胞减少小鼠模型中的竞争性感染指数，测试了 PvdI 在致病性中的作用。获得的数据证实了 PvdI 在毒力和铁吸收方面的重要性。 (C) 2000 年由 Elsevier Science B.V. 代表欧洲微生物学会联合会出版。
Novel putative pyoverdine synthetase pvdIJK genes were found upstream of pvdD in the 6.2-Mb chromosome of Pseudomonas aeruginosa strain PAO1. These genes formed a locus implicated in pyoverdine biosynthesis. Sequence analysis showed that the product of these genes shared 43%, 60% and 57% identity with PvdD. PvdIJK are thought to be implicated in synthesis of pyoverdine, a siderophore chelating Fe3+. A pvdI mutant was obtained by gene disruption mutagenesis and confirmed by Southern hybridization. The pvdI mutant produced gave no significant growth on solid media supplemented with the iron chelator 2,2-dipyridyl; while the PvdI(-) phenotype abolished pyoverdine fluorescence. The role of PvdI in pathogenicity was tested by measuring the in vivo growth of P. aeruginosa wild-type and mutant strains in a chronic lung infection rat model, and by measuring the competitive infectivity index into a neutropenic mice model. The data obtained confirmed the importance of PvdI in virulence and iron uptake. (C) 2000 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.