Ontogeny of phenylethanolamine N‐methyltransferase‐ and tyrosine hydroxylase‐like immunoreactivity in presumptive adrenaline neurones of the foetal rat central nervous system
Ontogeny of phenylethanolamine N‐methyltransferase‐ and tyrosine hydroxylase‐like immunoreactivity in presumptive adrenaline neurones of the foetal rat central nervous system
复制标题
胎鼠中枢神经系统假定肾上腺素神经元中苯乙醇胺 N-甲基转移酶和酪氨酸羟化酶样免疫反应性的个体发育
DOI：
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
T. Hökfelt
中科院分区：
文献类型：
--
作者：
G. Foster;M. Schultzberg;M. Goldstein;T. Hökfelt
研究了胎儿大鼠中枢神经系统中苯乙醇胺N‐甲基转移酶（PNMT）‐和酪氨酸羟化酶（TH）‐样免疫反应性（LI）的出现。间接免疫荧光法检测PNMT和TH抗体。注意力集中在假定含有肾上腺素的神经细胞体或纤维的区域，并使用洗脱抑制技术，可以分析神经元是否含有PNMT - LI， TH - LI，或两者兼有。PNMT免疫反应神经元在妊娠第13天首次可见，位于延髓腹外侧和延髓背侧，可能与C1和C2组相对应。这一阶段这些区域阳性细胞体的数量和荧光染色的强度与出生后1天观察到的数量和强度没有什么不同。第16天，PNMT阳性细胞首次出现在延髓背侧吻侧-桥尾侧中线区域，与内侧纵束相关。这些细胞可能组成C3细胞群。妊娠第13天，在髓质中可见许多PNMT免疫反应纤维，并在中脑屈曲周围呈上升束，在脊髓周围呈下降束。随着胎龄的增加，束的范围增加，因此在18至19天，在脑室周围和下丘脑外侧区域，以及在出生后第1天，在室旁核，间隔和胸脊髓，可以看到密集的PNMT免疫反应性静脉曲张纤维网。出生后第1天，在杏仁核中观察到稀疏的纤维丛。
The appearance of phenylethanolamine N‐methyltransferase (PNMT)‐and tyrosine hydroxylase (TH)‐like immunoreactivity (LI) in the foetal rat central nervous system has been investigated. Antibodies raised against PNMT and TH were used in an indirect immunofluorescence method. Attention was focussed on areas containing putative adrenaline‐containing nerve cell bodies or fibres and, using an elution‐restaining technique, it was possible to analyze whether neurones contained PNMT‐LI, TH‐LI, or both. PNMT‐immunoreactive neurones could first be visualized on day 13 of gestation, in the ventrolateral and dorsal medulla oblongata, and probably corresponding to those of the C1 and C2 groups. The number of positive cell bodies and the intensity of their fluorescent staining in these areas were not dissimilar at this stage to the number and intensity observed at 1 day postnatal, the final age studied. At day 16, PNMT‐positive cells were observed for the first time in midline areas of the rostral dorsal medulla oblongata‐caudal pons, associated with the medial longitudinal fasciculus. These cells probably composed the C3 cell group. Many PNMT‐immunoreactive fibres could be seen at day 13 of gestation, in the medulla, and coursing both in an ascending bundle around the mesencephalic flexure and in a descending bundle toward the spinal cord. The extent of the bundles increased with gestational age, such that dense meshworks of PNMT‐immunoreactive varicose fibres were visible ventral to the aqueductus Sylvii, and the periventricular and lateral regions of the hypothalamus by days 18 to 19, and in the paraventricular nucleus, the septum, and the thoracic spinal cord by day 1 after birth. A sparser fibre plexus was observed in the amygdala at day 1 postnatal.
对苯二胺可延缓免疫荧光的褪色并增强其强度。
DOI：
10.1177/31.6.6341464
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Platt,JL;Michael,AF
通讯作者：
Michael,AF
苯乙醇胺 N-甲基转移酶的物种特异性电荷形式。
影响因子：
2.9
作者：
Park,DH;Joh,TH
通讯作者：
Joh,TH