Transport of Egg White ACE-Inhibitory Peptide, Gln-Ile-Gly-Leu-Phe, in Human Intestinal Caco-2 Cell Monolayers with Cytoprotective Effect
Transport of Egg White ACE-Inhibitory Peptide, Gln-Ile-Gly-Leu-Phe, in Human Intestinal Caco-2 Cell Monolayers with Cytoprotective Effect
复制标题
蛋白 ACE 抑制肽 Gln-Ile-Gly-Leu-Phe 在人肠 Caco-2 细胞单层中的转运具有细胞保护作用
DOI：
10.1021/jf405639w
复制
发表时间：
2014-04-09
影响因子：
6.1
通讯作者：
Liu, Jingbo
中科院分区：
文献类型：
--
作者：
Ding, Long;Zhang, Yan;Liu, Jingbo
本研究的目的是研究一种来源于鸡蛋白色卵白蛋白的ACE抑制肽Gln-lle-GlyLeu-Phe（QIGLF）在人小肠Caco-2细胞单层中的跨上皮转运和细胞保护作用。结果表明，CZIGLF可通过Caco-2细胞单层完整吸收，Papp值为（9.11 ± 0.19）× 10（-7）cm/s（转运动力学参数：Km，32.37 ± 12.59 mM; V-max，1.23 ± 0.49 mu M/min cm（2））。叠氮化钠和Gly-Pro（分别为ATP合成抑制剂和肽转运蛋白1（PepT 1）底物）均未显著降低QIGLF的转运，表明QIGLF的转运不是能量依赖性和载体介导的。此外，转胞吞作用抑制剂渥曼青霉素对QIGLF的转运影响不大，表明内吞作用不参与QIGLF的转运。然而，QIGLF的运输显着增加的存在下，细胞松弛素II，紧密连接破坏，表明通过紧密连接的细胞旁运输的主要运输机制为完整的QIGLF跨Caco-2细胞单层。此外，QIGLF加入到Caco-2细胞中，然后加入H2 O2，并表现出显着的细胞保护作用，Caco-2细胞对H2 O2诱导的氧化应激。
The purpose of this study was to investigate the transepithelial transport and cytoprotective effect of Gln-lle-GlyLeu-Phe (QIGLF), an ACE-inhibitory peptide derived from egg white ovalbumin, in human intestinal Caco-2 cell monolayers. The results showed that CZIGLF could be absorbed intact through Caco-2 cell monolayers with a Papp value of (9.11 0.19) x 10(-7) cm/s (transport kinetic parameters: K-m, 32.37 +/- 12.59 mM; V-max, 1.23 +/- 0.49 mu M/min cm(2)). The transport was not significantly decreased by sodium azide and Gly-Pro, an ATP synthesis inhibitor and a peptide transporter 1 (PepT1) substrate, respectively, suggesting that transport of QIGLF was not energy-dependent and carrier-mediated. In addition, wortmannin, a transcytosis inhibitor, had little effect on the transport, suggesting that endocytosis was not involved in the transport of QIGLF. However, the transport of QIGLF was increased significantly in the presence of cytochalasin ll, a tight junction disruptor, suggesting that paracellular transport via tight junctions was the major transport mechanism for intact QIGLF across Caco-2 cell monolayers. Moreover, QIGLF was added to Caco-2 cells followed by addition of H2O2,, and exhibited significant cytoprotective effect in Caco-2 cells against oxidative stress induced by H2O2.