Clock controls circadian period in isolated suprachiasmatic nucleus neurons
Clock controls circadian period in isolated suprachiasmatic nucleus neurons
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DOI：
10.1038/3708
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发表时间：
1998-12-01
影响因子：
25
通讯作者：
Block, GD
中科院分区：
文献类型：
--
作者：
Herzog, ED;Takahashi, JS;Block, GD
视交叉上核(SCN)是哺乳动物主要的昼夜节律起搏器，时钟基因是调节昼夜节律的分子之一。在这里，我们研究了从时钟突变小鼠分离的SCN神经元的放电模式。长期的多电极记录显示，杂合的时钟突变神经元的周期延长，纯合子的时钟神经元是心律失常的，与对动物运动活动的影响平行。此外，分散体中的细胞比外植体中的细胞表达更广泛的周期和时相关系。这些结果表明，时钟基因在单个SCN细胞的昼夜节律中是必需的，SCN内的一种机制使神经元同步，并限制了表达的昼夜节律周期的范围。
The suprachiasmatic nucleus (SCN) is the master circadian pacemaker in mammals, and one molecular regulator of circadian rhythms is the Clock gene. Here we studied the discharge patterns of SCN neurons isolated from Clock mutant mice. Long-term, multielectrode recordings showed that heterozygous Clock mutant neurons have,lengthened periods and that homozygous Clock neurons are arrhythmic, paralleling the effects on locomotor activity in the animal. In addition, cells in dispersals expressed a wider range of periods and phase relationships than cells in explants. These results suggest that the Clock gene is required for circadian rhythmicity in individual SCN cells and that a mechanism within the SCN synchronizes neurons and restricts the range of expressed circadian periods.