Sequence and regional distribution of the mRNA encoding the alpha 2 polypeptide of rat gamma-aminobutyric acidA receptors.
Sequence and regional distribution of the mRNA encoding the alpha 2 polypeptide of rat gamma-aminobutyric acidA receptors.
复制标题
编码大鼠γ-氨基丁酸A受体α2多肽的mRNA的序列和区域分布。
DOI：
10.1111/j.1471-4159.1991.tb02072.x
复制
发表时间：
1991
影响因子：
4.7
通讯作者：
Tobin,AJ
中科院分区：
文献类型：
--
作者：
Khrestchatisky,M;MacLennan,AJ;Tillakaratne,NJ;Chiang,MY;Tobin,AJ
来自大鼠海马 cDNA 文库的 cDNA 编码 γ-氨基丁酸 (GABA)/苯二氮卓 (BZ) 受体的 α 多肽同工型。其推导的氨基酸序列与牛GABAA受体的α2多肽的氨基酸序列有96%的同一性。该多肽具有所有先前报道的 GABAA 受体 α 多肽所共有的特征，并且与先前描述的大鼠 α 多肽具有 71-76% 的同一性。大多数差异在于假定的细胞外和细胞内结构域。在 Northern 印迹中，α2cDNA 检测到两种 mRNA，它们存在于皮层、海马体和纹状体中，这些大脑区域富含药理学定义的“BZ II 型”受体。其他工作人员之前已经表明，GABAA 受体的 α 多肽在很大程度上决定了重建受体的 BZ 结合特性。 α2mRNA在大鼠脑中的分布表明α2亚基确实可能参与BZ II型受体。
A cDNA from a rat hippocampal cDNA library encodes an isoform of the α polypeptide of the γ‐aminobutyric acid (GABA)/benzodiazepine (BZ) receptor. Its deduced amino acid sequence is 96% identical to that of the α2polypeptide of the bovine GABAAreceptor. The polypeptide has features shared by all previously reported GABAAreceptor α polypeptides and shares 71–76% identity with previously described rat α polypeptides. Most of the differences lie in the presumed extracellular and intracellular domains. On Northern blots, the α2cDNA detects two mRNAs, which are found in cortex, hippocampus, and striatum, brain regions enriched in pharmacologically defined “BZ type II” receptors. Other workers have previously shown that the α polypeptides of the GABAAreceptor largely determine the BZ binding properties of reconstituted receptors. The distribution of α2mRNAs in rat brain suggests that the α2subunit may indeed be involved in the BZ type II receptors.