PURIFICATION OF PLANT CALMODULIN BY FLUPHENAZINE-SEPHAROSE AFFINITY CHROMATOGRAPHY
PURIFICATION OF PLANT CALMODULIN BY FLUPHENAZINE-SEPHAROSE AFFINITY CHROMATOGRAPHY
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DOI：
10.1016/0006-291x(79)91931-4
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
CORMIER, MJ
中科院分区：
文献类型：
--
作者：
CHARBONNEAU, H;CORMIER, MJ
利用吩噻嗪药物与钙调蛋白的 Ca 依赖性结合，开发了一种基于氟奋乃静-琼脂糖亲和层析的钙调蛋白快速纯化方法。钙调蛋白可通过钙离子依赖性结合氟奋乃静-琼脂糖，轻松纯化来自植物 [豌豆和花生]、真菌 [双孢蘑菇]、猪脑和腔肠动物海肾的钙调蛋白。
The Ca-dependent binding of phenothiazine drugs to calmodulin was utilized to develop a rapid purification procedure for calmodulin based on fluphenazine-Sepharose affinity chromatography. Calmodulin from plants [Pisum sativum and Arachis hypogaea], a fungus [Agaricus bisporus], porcine brain and the coelenterate, Renilla reniformis, were easily purified by the Ca-dependent binding of calmodulin to fluphenazine-Sepharose.