A comparative evaluation of various invasion assays testing colon carcinoma cell lines.
A comparative evaluation of various invasion assays testing colon carcinoma cell lines.
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DOI：
10.1038/sj.bjc.6690790
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发表时间：
1999-11
影响因子：
8.8
通讯作者：
Bosman FT
中科院分区：
文献类型：
--
作者：
de Both NJ;Vermey M;Dinjens WN;Bosman FT
对不同的结肠癌细胞株进行了不同的侵袭实验，即侵袭Matrigel、侵袭融合成纤维细胞层和侵袭鸡心组织。此外，还测定了裸鼠的侵袭能力和转移潜能。所使用的结肠癌细胞系为人细胞系Caco-2、SW-480、SW-620和HT-29，以及小鼠细胞系Colon-26和-38。没有人的结肠癌细胞通过涂有Matrigel的多孔膜迁移；在小鼠细胞系中，只有Colon-26迁移了。当在Matrigel和培养液的混合物中孵育时，非侵袭细胞形成椭圆形培养物，而侵袭细胞则显示星状突起。只有SW-480和SW-620的异形(上皮样和星状)细胞和Colon-26的梭形细胞明显侵袭融合的皮肤和结肠成纤维细胞以及鸡心组织。然而，当移植到裸鼠和同基因小鼠的盲肠内时，除Caco-2细胞外，所有被测试的株系都是侵袭性的。我们的结论是，衡量肿瘤细胞侵袭能力的体外测试结果在很大程度上取决于所使用的测试系统。体外侵袭能力与细胞具有梭形细胞形态、波形蛋白表达和E-钙粘附素下调密切相关。相反，具有上皮样表型的HT-29和Colon-38细胞在体内具有明显的侵袭性和转移性，但在体外不是。©1999癌症研究活动
Various colon carcinoma cell lines were tested in different invasion assays, i.e. invasion into Matrigel, into confluent fibroblast layers and into chicken heart tissue. Furthermore, invasive capacity and metastatic potential were determined in nude mice. The colon carcinoma cells used were the human cell lines Caco-2, SW-480, SW-620 and HT-29, and the murine lines Colon-26 and -38. None of the human colon carcinoma cells migrated through porous membranes coated with Matrigel; of the murine lines, only Colon-26 did. When incubated in a mixture of Matrigel and culture medium non-invading cells formed spheroid cultures, whereas invading cells showed a stellate outgrowth. Only the heterogeneously shaped (epithelioid and stellate) cells of SW-480 and SW-620 and the spindle-shaped cells of Colon-26 invaded clearly confluent skin and colon fibroblasts as well as chicken heart tissue. However, when transplanted into the caecum of nude and syngeneic mice, all the lines tested were invasive with the exception of Caco-2 cells. We conclude that the outcome of in vitro tests measuring the invasive capacity of neoplastic cells is largely dependent on the test system used. Invasive capacity in vitro is strongly correlated with cells having a spindle cell shape, vimentin expression and E-cadherin down regulation. In contrast, HT-29 and Colon-38 cells having an epithelioid phenotype were clearly invasive and metastatic in vivo, but not in vitro. © 1999 Cancer Research Campaign