Superoxide dismutase 3 facilitates the chondrogenesis of bone marrow-derived mesenchymal stem cells
Superoxide dismutase 3 facilitates the chondrogenesis of bone marrow-derived mesenchymal stem cells
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超氧化物歧化酶3促进骨髓间充质干细胞的软骨形成
DOI：
10.1016/j.bbrc.2019.01.042
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发表时间：
2019-02-19
影响因子：
3.1
通讯作者：
Ao, Yingfang
中科院分区：
文献类型：
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作者：
Shi, Yuanyuan;Hu, Xiaoqing;Ao, Yingfang
关节软骨缺损被认为是一个主要的临床问题，因为它们不能自行愈合。迄今为止，基于骨髓间充质干细胞（BMSCs）的治疗已被广泛应用于软骨修复。然而，纤维软骨往往产生BMSC治疗后，因此，有一个迫切需要刺激和维持BMSC软骨分化。超氧化物歧化酶3（SOD 3）在软骨形成中的具体作用尚不清楚，因此，本研究旨在阐明SOD 3是否可以促进BMSCs的软骨分化。我们首先使用平板培养评估了BMSCs软骨形成过程中SOD 3蛋白水平。然后，我们使用敲低或过表达实验来测试SOD 3是否可以促进BMSC的软骨形成。在BMSCs软骨形成过程中观察到SOD 3蛋白水平增加。SOD 3敲低抑制II型胶原α 1链（COL 2A 1）、聚集蛋白聚糖（ACAN）和SRY-盒9（SOX 9）表达。SOD 3的过表达增加了软骨形成标志物（COL 2A 1、ACAN和SOX 9）的水平。当SOD 3被敲低时，观察到超氧阴离子升高。结论：SOD 3可以促进骨髓基质细胞的软骨形成，促进软骨再生。(C)2019爱思唯尔公司All rights reserved.
Articular cartilage defects are considered a major clinical problem because they cannot heal by themselves. To date, bone marrow-derived mesenchymal stem cells (BMSCs)-based therapy has been widely applied for cartilage repair. However, fibrocartilage was often generated after BMSC therapy; therefore, there is an urgent need to stimulate and maintain BMSCs chondrogenic differentiation. The specific role of superoxide dismutase 3 (SOD3) in chondrogenesis is unknown; therefore, the present study aimed to clarify whether SOD3 could facilitate the chondrogenic differentiation of BMSCs. We first evaluated SOD3 protein levels during chondrogenesis of BMSCs using plate cultures. We then tested whether SOD3 could facilitate chondrogenesis of BMSCs using knockdown or overexpression experiments. Increased SOD3 protein levels were observed during BMSCs chondrogenesis. SOD3 knockdown inhibited collagen type II alpha 1 chain (COL2A1), aggrecan (ACAN), and SRY-box 9 (SOX9) expression. Overexpression of SOD3 increased the levels of chondrogenesis markers (COL2A1, ACAN, and SOX9). Elevated superoxide anions were observed when SOD3 was knocked down. We concluded that SOD3 could facilitate chondrogenesis of BMSCs to improve cartilage regeneration. (C) 2019 Elsevier Inc. All rights reserved.