Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi.
Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi.
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DOI：
10.1155/s0962935192000450
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发表时间：
1992
影响因子：
4.6
通讯作者：
Silva, W D
中科院分区：
文献类型：
--
作者：
Kipnis, T L;Tambourgi, D V;Alves, M J;Silva, W D
抗克氏锥虫或其IgG和IgM组分的小鼠多克隆抗血清和五种单克隆抗体（两种IgM、两种IgG 1和一种IgG 2a）识别并联合收割机结合寄生虫的锥鞭毛体形式的膜组分，如通过免疫荧光显示的。尽管所有这些抗体都能使锥虫鞭毛体敏感并使其激活补体（C）系统，如通过总C、C4、B和C3的消耗所测量的，但只有多克隆抗血清或其IgG、IgM和Fab B μ片段能够通过替代C途径诱导锥虫溶解。
A Mouse polyclonal antiserum against Trypanosoma cruzi or its IgG and IgM fractions and five monoclonal antibodies (two IgM, two IgG1 and one IgG2a) recognize and combine with membrane components of trypomastigote forms of the parasite as revealed by immunofluorescence. Although all these antibodies sensitize trypomastigotes and prepare them to activate the complement (C) system, as measured by consumption of total C, C4, B and C3, only the polyclonal antiserum or its IgG, IgM and Fabμ fragments were able to induce trypanosome lysis by the alternative C pathway.